Assessment of brain injury in cattle with Theileria annulata: Neuron-specific biomarkers, inflammation, oxidative stress and apoptosis

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal Pub Date : 2024-11-22 DOI:10.1016/j.tvjl.2024.106269

M. Uztimür , C.N. Ünal , M.B. Dörtbudak , R. Fırat , A.İ. Ekinci

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引用次数: 0

Abstract

This study aimed to investigate selected brain-specific biomarkers in cattle with tropical theileriosis caused by Theileria annulata (T. annulata) and to evaluate their diagnostic and prognostic significance. The study group consisted of 25 cattle naturally infected with T. annulata, while the control group consisted of 10 healthy cattle. Animals with T. annulata were classified according to hematocrit (HCT) value as severe anemia group 1 (n:12) with HCT ≤12 and moderate anemia group 2 (n:13) with HCT between 13 and 24. Histopathological and immunohistochemical examinations of the brain tissue were performed in 10 nonsurvivor cattle. Serum calcium-binding protein B (S100B), glial fibrillary acidic protein (GFAP), tau protein and ubiquitin C-terminal hydrolase-1 (UCHL-1) concentrations in brain injury were measured using bovine-specific ELISA kits. S100B, GFAP, and Tau concentrations of cattle in the T. annulata group were found to be significantly higher than the control group (P<0.001). Brain specific biomarkers showed significant correlations with erythrocyte count, HCT and bilirubin. The presence of brain damage was confirmed by histopathological and immunohistochemical techniques. The results of ROC analysis showed that S100B with AUC value of 0.88 and GFAP with an AUC value of 0.82 were significant prognostic indicators. Additionally, S100B, GFAP and Tau showed significant diagnostic performance with an AUC value of 0.88, 0.92 and 0.86, respectively. In conclusion, brain-specific biomarkers can be used as diagnostic and prognostic markers in the assessment of brain damage in cattle naturally infected with T. annulata.

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评估环状丝虫病牛的脑损伤：神经元特异性生物标志物、炎症和细胞凋亡

本研究旨在调查由环状沙雷氏菌（T. annulata）引起的热带沙雷氏菌病牛的某些脑特异性生物标志物，并评估其诊断和预后意义。研究组由 25 头自然感染环状丝虫病的牛组成，对照组由 10 头健康牛组成。根据血细胞比容（HCT）值将感染环斑蓟马的牛分为重度贫血 1 组（12 头）和中度贫血 2 组（13 头），前者的 HCT 值小于 12，后者的 HCT 值介于 13 和 24 之间。对 10 头非存活牛的脑组织进行了组织病理学和免疫组化检查。使用牛特异性酶联免疫吸附试剂盒测定了脑损伤中血清钙结合蛋白 B (S100B)、胶质纤维酸性蛋白 (GFAP)、tau 蛋白和泛素 C 端水解酶-1 (UCHL-1)的浓度。结果发现，T. annulata 组牛的 S100B、GFAP 和 Tau 蛋白浓度明显高于对照组（P<0.05）。

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来源期刊

Veterinary journal 农林科学-兽医学

CiteScore

4.10

自引率

4.50%

发文量

审稿时长

40 days

期刊介绍： The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.