Development of point-of-care immunodiagnostic test for Taenia solium cysticercosis in pigs.

IF 2.2 3区农林科学 Q1 VETERINARY SCIENCES

Research in veterinary science Pub Date : 2024-11-20 DOI:10.1016/j.rvsc.2024.105466

Haranahally Vasanthachar Manjunathachar, Buddhi Chandrasekaran Saravanan, Chitra Joshi, Aquil Mohmad, M Arvind, Siju Susan Jacob, M Sankar

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Abstract

Cysticercosis, caused by the larval stage of Taenia solium, is a major zoonotic parasitic disease with a global impact, heightened by immigration and transboundary activities. Recognizing its importance, the World Health Organization has prioritized eliminating taeniasis/cysticercosis by 2030, designating it as a priority neglected tropical disease (NTD). Accurate diagnosis is essential for effective disease control, yet traditional methods often fall short due to low sensitivity of the test and the need for specialized laboratory setups. This study focuses on developing a recombinant Ag2 (rAg2)-based dot blot assay to detect T. solium cysticercosis in pigs. The rAg2 antigen was successfully expressed and purified, with immunoreactivity confirmed against sera from naturally infected pigs. The dot blot test evaluated against serological gold-standard enzyme-linked immunotransfer blot (EITB) assay, demonstrated preliminary laboratory sensitivity (89 %, 95 % CI: 68.6-97.1) and specificity (100 %, 95 % CI: 97.6 - 100), highlighting its potential as a reliable alternative diagnostic method. Cost-effectiveness and minimal laboratory requirements make it particularly suited for field use in resource-limited settings.

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开发针对猪肺结核囊尾蚴病的护理点免疫诊断试验。

由蛔虫幼虫阶段引起的囊尾蚴病是一种主要的人畜共患寄生虫病，具有全球性影响，并因移民和跨境活动而加剧。世界卫生组织认识到这一疾病的重要性，已将在2030年前消灭癞皮病/囊尾蚴病列为优先事项，并将其指定为被忽视的热带疾病（NTD）。准确的诊断对有效控制疾病至关重要，但传统方法往往因检测灵敏度低和需要专门的实验室设备而达不到预期效果。本研究的重点是开发一种基于重组Ag2（rAg2）的点印迹检测法，用于检测猪的梭状芽孢杆菌囊尾蚴病。rAg2抗原已成功表达和纯化，对自然感染猪血清的免疫反应已得到证实。点印迹试验与血清学黄金标准酶联免疫转移印迹（EITB）测定进行了评估，初步显示了实验室灵敏度（89 %，95 % CI：68.6-97.1）和特异性（100 %，95 % CI：97.6-100），突出了其作为可靠替代诊断方法的潜力。成本效益高、实验室要求低，因此特别适合在资源有限的环境中实地使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in veterinary science 农林科学-兽医学

CiteScore

4.40

自引率

4.20%

发文量

312

审稿时长

75 days

期刊介绍： Research in Veterinary Science is an International multi-disciplinary journal publishing original articles, reviews and short communications of a high scientific and ethical standard in all aspects of veterinary and biomedical research. The primary aim of the journal is to inform veterinary and biomedical scientists of significant advances in veterinary and related research through prompt publication and dissemination. Secondly, the journal aims to provide a general multi-disciplinary forum for discussion and debate of news and issues concerning veterinary science. Thirdly, to promote the dissemination of knowledge to a broader range of professions, globally. High quality papers on all species of animals are considered, particularly those considered to be of high scientific importance and originality, and with interdisciplinary interest. The journal encourages papers providing results that have clear implications for understanding disease pathogenesis and for the development of control measures or treatments, as well as those dealing with a comparative biomedical approach, which represents a substantial improvement to animal and human health. Studies without a robust scientific hypothesis or that are preliminary, or of weak originality, as well as negative results, are not appropriate for the journal. Furthermore, observational approaches, case studies or field reports lacking an advancement in general knowledge do not fall within the scope of the journal.