Efficacy of photoClO2 against two human norovirus surrogates and Clostridioides difficile endospores on stainless steel and nylon carpet.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2024-11-23 DOI:10.1093/jambio/lxae294

Jinge Huang, Jacob Adams, Charles Pettigrew, Angela Fraser, Xiuping Jiang

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引用次数: 0

Abstract

Aims: Determine efficacy of an aqueous photocatalytic disinfection system, photoClO2, against two human norovirus surrogates [feline calicivirus (FCV) and Tulane virus (TuV)] and Clostridioides difficile endospores on stainless steel and nylon carpet.

Methods and results: The photoClO2 system was first optimized with 1% sodium chlorite (NaClO2) and 10 ppm Eosin Y to produce 60.64 ppm ClO2/min in a 4.5×4.5 cm2 area. It was then tested against FCV, TuV, and C. difficile endospores on stainless steel and nylon carpet with two different backings. On stainless steel, photoClO2 achieved a >5 log10 plaque-forming unit (PFU) reduction of FCV in 45 min, >3 log10 median tissue culture infectious dose (TCID50) reduction of TuV in 60 min, and 1.3 log10 colony-forming unit (CFU) reduction of C. difficile endospores in 120 min. Under indoor lighting conditions, photoClO2 achieved a 4.3 log10 PFU reduction of FCV and 1.4 log10 TCID50 reduction of TuV on stainless steel after 120 min. Further, photoClO2 achieved a 2.9 log10 PFU reduction of FCV and 2.5 log10 TCID50 reduction of TuV on nylon carpet with waterproof backing in 60 min, which was higher than carpet with water-permeable backing (1.3 log10 PFU and 1.1 log10 TCID50 reduction, respectively).

Conclusion: ClO2 production rate of the photoClO2 system was influenced by light distribution, while disinfection efficacy was affected by light intensity, surface characteristics, and target microorganisms. PhotoClO2 was efficacious in inactivating both human norovirus surrogates on stainless steel and nylon carpet. Efficacy against C. difficile endospores was limited.

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光二氧化氯对不锈钢和尼龙地毯上两种人类诺如病毒代用品和艰难梭菌内孢子的功效。

目的：确定水基光催化消毒系统（photoClO2）对不锈钢和尼龙地毯上的两种人类诺如病毒代用品[猫嗜热病毒（FCV）和杜兰病毒（TuV）]和艰难梭菌内孢子的功效：首先使用 1% 亚氯酸钠 (NaClO2) 和 10 ppm 曙红 Y 对光二氧化氯系统进行了优化，在 4.5×4.5 平方厘米的区域内产生 60.64 ppm ClO2/分钟。然后在不锈钢和尼龙地毯上对 FCV、TuV 和艰难梭菌内孢子进行了测试。在不锈钢上，光二氧化氯能在 45 分钟内使 FCV 的斑块形成单位 (PFU) 降低 >5 log10，在 60 分钟内使 TuV 的组织培养感染剂量中值 (TCID50) 降低 >3 log10，在 120 分钟内使艰难梭菌内孢子的菌落形成单位 (CFU) 降低 1.3 log10。在室内照明条件下，120 分钟后，光二氧化氯可使不锈钢上的 FCV 减少 4.3 log10 PFU，TuV 减少 1.4 log10 TCID50。此外，60 分钟后，光二氧化氯可使防水背衬的尼龙地毯上的 FCV 减少 2.9 log10 PFU，TuV 减少 2.5 log10 TCID50，高于透水背衬的地毯（分别减少 1.3 log10 PFU 和 1.1 log10 TCID50）：结论：光ClO2 系统的 ClO2 生成率受光照分布的影响，而消毒效果则受光照强度、表面特征和目标微生物的影响。光二氧化氯能有效灭活不锈钢和尼龙地毯上的诺如病毒代用品。对艰难梭菌内孢子的功效有限。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.