Application of magnetic bead-assisted liquid chromatography-tandem mass spectrometry method for analyzing serum bile acid profiles: Advancing toward automated mass spectrometry

IF 3.2 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2024-11-22 DOI:10.1016/j.cca.2024.120058

Yu Zhou , Jing Du , Yamei Liu , Jinfei Ma , Xiaofen Yuan , Jun Xia

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引用次数: 0

Abstract

Background

Magnetic bead-assisted extraction technology offers potential for automating mass spectrometry processes, yet research in this area remains limited. Here, we developed and validated a magnetic bead extraction method to simultaneously analyze 13 bile acid profiles in human serum.

Methods

A magnetic bead-assisted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for serum bile acid profiles. The linearity, accuracy, precision, matrix effect, carryover, stability test and interference test of the LC-MS/MS method were assessed. Comparative analysis involving 20 pregnant women evaluated different methodologies for diagnosing and monitoring intrahepatic cholestasis of pregnancy (ICP).

Results

The magnetic bead extraction method demonstrated analytical performance comparable to the traditional protein precipitation method. The method exhibited linear responses across the clinical reference interval for all 13 bile acids. Meanwhile, the recovery rates for bile acid analytes ranged from 85.14 to 114.43%, and the repeatability was within 1.68 to 10.83%, meeting acceptance criteria. Our magnetic bead-assisted extraction method showed no obvious matrix effects and carryover effects. The extracted specimens remained stable in the autosampler for 24 h, and the interference test suggested the bile acid profile results would not be significantly affected under different interfering substances. Furthermore, comparative studies with traditional protein precipitation method showed comparable results, and our method exhibited robust clinical consistency in monitoring the efficacy of ICP patients.

Conclusions

We developed a straightforward magnetic bead extraction method coupled with LC-MS/MS for bile acid profiles. It can be applied to the clinical routine diagnosis of ICP and facilitate the automated mass spectrometry processes.

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应用磁珠辅助液相色谱-串联质谱法分析血清胆汁酸谱：向自动质谱法迈进。

背景：磁珠辅助萃取技术为质谱分析过程的自动化提供了可能，但这一领域的研究仍然有限。在此，我们开发并验证了一种磁珠萃取方法，可同时分析人血清中的 13 种胆汁酸：方法：建立了一种磁珠辅助液相色谱-串联质谱（LC-MS/MS）分析血清胆汁酸的方法。评估了 LC-MS/MS 方法的线性、准确度、精密度、基质效应、携带、稳定性测试和干扰测试。由 20 名孕妇参与的比较分析评估了诊断和监测妊娠期肝内胆汁淤积症（ICP）的不同方法：结果：磁珠提取法的分析性能与传统的蛋白沉淀法相当。该方法对所有 13 种胆汁酸在临床参考区间内均呈线性反应。同时，胆汁酸分析物的回收率在 85.14% 至 114.43% 之间，重复性在 1.68% 至 10.83% 之间，符合接受标准。磁珠辅助萃取法没有明显的基质效应和携带效应。提取的标本在自动进样器中可保持稳定 24 小时，干扰试验表明，在不同干扰物质的作用下，胆汁酸图谱结果不会受到明显影响。此外，与传统蛋白沉淀法的比较研究显示结果相当，我们的方法在监测 ICP 患者疗效方面具有很强的临床一致性：结论：我们开发了一种简便的磁珠萃取法与 LC-MS/MS 联用的胆汁酸谱分析方法。结论：我们开发出了一种简便的磁珠提取法与 LC-MS/MS 联用的胆汁酸谱分析方法，该方法可应用于 ICP 的临床常规诊断，并有助于自动质谱分析过程。

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来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.