Au@Ag hollow nanoshells-based SERS integrated microfluidic chip as a sample-to-answer platform for the ultra-sensitive detection of geosmin

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-23 DOI:10.1016/j.aca.2024.343471

Yuting Gong , Dong Li , Min Chen , Anhui Lin , Quansheng Chen , Xiaomei Chen

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引用次数: 0

Abstract

Background

Geosmin (GSM) can compromise the immune systems of aquatic organisms, rendering them more vulnerable to viral and bacterial infections, thereby adversely affecting their growth, reproduction, yield, and quality. Given the relatively low odor thresholds of GSM, there is a critical demand for the development of a highly sensitive and rapid detection method. According to the principle of Surface-enhanced Raman spectroscopy (SERS), localized surface plasmon resonance (LSPR) greatly enhances the Raman signals of adsorbed molecules. To date, no study has reported the application of SERS to detect GSM.

Results

Dual-metal nanomaterials with hollow structures have been proven to provide a large surface area and heightened localized surface plasmon resonance, thereby enhancing the sensitivity of Raman signals. In this study, a sample-to-answer platform was constructed by integrating Au@Ag hollow nanoshells (HNSs)-based SERS and a microfluidic chip for the sensitive, fast, and direct determination of GSM. Under 532 nm excitation, GSM exhibit Raman peaks on the SERS-active Au@Ag HNSs, and there is a relationship between the peak intensity and the concentration of GSM. Owing to the integration of the microfluidic chip, only microliters of reagent are required, and the test results can be achieved within 4 min. The constructed sample-to-answer platform showed a good linear response to GSM in the range of 1 ng/L–1 mg/L, with a detection limit of 0.16 ng/L. An optimal calibration model is established by combining stoichiometric algorithms.

Significance

This work realized the ultra-highly sensitive and fast determination of GSM based on SERS-active Au@Ag HNSs, which provides new insights and is very promising for the on-site monitoring of earthy odors. Our study makes a significant contribution to the literature because the developed SERS-based detection method does not require labels or biomaterials, providing the possibility of highly sensitive and on-site testing of contaminants in food and the environment.

Abstract Image

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基于 Au@Ag 中空纳米壳的 SERS 集成微流控芯片作为超灵敏地奥司明检测的样品-应答平台

背景草甘膦（GSM）会损害水生生物的免疫系统，使其更容易受到病毒和细菌感染，从而对其生长、繁殖、产量和质量产生不利影响。由于 GSM 的气味阈值相对较低，因此亟需开发一种高灵敏度的快速检测方法。根据表面增强拉曼光谱（SERS）原理，局部表面等离子体共振（LSPR）可大大增强吸附分子的拉曼信号。结果具有中空结构的双金属纳米材料已被证实具有较大的表面积和更高的局部表面等离子体共振，从而提高了拉曼信号的灵敏度。本研究通过将基于 Au@Ag 中空纳米壳（HNSs）的 SERS 与微流控芯片相结合，构建了一个样品到答案的平台，用于灵敏、快速、直接地测定 GSM。在 532 纳米的激发下，GSM 在具有 SERS 活性的 Au@Ag HNSs 上显示出拉曼峰，峰强度与 GSM 的浓度之间存在一定的关系。由于集成了微流控芯片，只需微升试剂，4 分钟内就能得到检测结果。所构建的样品-应答平台对 1 ng/L-1 mg/L 范围内的 GSM 具有良好的线性响应，检测限为 0.16 ng/L。这项工作基于 SERS 活性 Au@Ag HNSs 实现了对 GSM 的超高灵敏度和快速测定，为现场监测泥土气味提供了新的视角和前景。我们的研究为文献做出了重大贡献，因为所开发的基于 SERS 的检测方法不需要标签或生物材料，为高灵敏度和现场检测食品和环境中的污染物提供了可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.