Exogenous application of melatonin and chitosan mitigate simulated microgravity stress in the Rocket (Eruca sativa L.) plant

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-14 DOI:10.1016/j.plaphy.2024.109294

Hilda Amiripour , Alireza Iranbakhsh , Sara Saadatmand , Fateme Mousavi , Zahra Oraghi Ardebili

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Abstract

Starting life in space and implementing spaceflight missions requires raising of plants in special conditions, where various stresses, including microgravity, are applied to plant. The use of stimulants is known as a promising effective approach that enhances plant resistance encountered a variety of abiotic stresses. In this study, the impact of two stimulants, melatonin and chitosan, in reducing negative effects of clinorotation on Rocket (Eruca sativa L.) seedlings was investigated from a physiological and biochemical point of view. For this purpose, a completely randomized experiment was designed where the treatments included control (without stimulants and normal gravity), melatonin (100 μM), chitosan (230 M), microgravity, microgravity + melatonin, and microgravity + chitosan. The results disclosed that the microgravity significantly impaired the plant growth and morphology, while exogenous application of melatonin and chitosan improved the plant growth parameters under stress conditions. Under microgravity, there was a reduction of 46.15% in shoot length (4.9 mm) and 41.44% in root length (4.7 mm) compared with the control (9.1 mm; 8.03 mm), respectively. Clinorotation led to a marked increment in the enzymes activity, wherein the POD, SOD and CAT activities increased by 75.13%, 72.67%, and 53.42%, respectively, compared with the control seedlings. In addition, supply of these two stimulants strengthened the scavenging of radial oxygen species and helped the plant to tolerate stress conditions, by activated the enzymatic and non-enzymatic systems. These results can pave the road for more studies and broad application of biological stimuli to overcome the space harsh environmental conditions by plants.

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褪黑素和壳聚糖的外源应用可减轻火箭（Eruca sativa L.）植物的模拟微重力胁迫。

开始太空生活和执行太空飞行任务需要在特殊条件下培育植物，在这种条件下植物会受到包括微重力在内的各种胁迫。众所周知，使用刺激剂是一种很有前景的有效方法，可增强植物对各种非生物胁迫的抵抗力。本研究从生理和生化角度研究了两种刺激剂（褪黑素和壳聚糖）对减少浮选对火箭（Eruca sativa L.）幼苗的负面影响的影响。为此，设计了一个完全随机的实验，处理包括对照组（无刺激物和正常重力）、褪黑素（100 μM）、壳聚糖（230 M）、微重力、微重力+褪黑素和微重力+壳聚糖。结果表明，微重力显著影响了植物的生长和形态，而外源施用褪黑素和壳聚糖则改善了胁迫条件下植物的生长参数。在微重力条件下，与对照组（9.1 毫米；8.03 毫米）相比，芽长（4.9 毫米）减少了 46.15%，根长（4.7 毫米）减少了 41.44%。浮选使酶活性显著提高，与对照苗相比，POD、SOD 和 CAT 活性分别提高了 75.13%、72.67% 和 53.42%。此外，提供这两种刺激剂还能通过激活酶和非酶系统，加强对径向氧物种的清除，帮助植物耐受胁迫条件。这些结果为植物克服空间恶劣环境条件的更多研究和生物刺激的广泛应用铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.