Re-sensitization of imipenem-resistant Pseudomonas aeruginosa and restoration of cephalosporins susceptibility in Enterobacteriaceae by recombinant Esterase B.

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wirongrong Whangsuk, Punyawee Dulyayangkul, Suvit Loprasert, James M Dubbs, Paiboon Vattanaviboon, Skorn Mongkolsuk
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引用次数: 0

Abstract

Sphingobium sp. SM42 Esterase B (EstB) is an enzyme with a dual function in degrading dibutyl phthalate and catalyzing the cleavage of the C-S bond in C3-sidechains of the dihydrothiazine ring of cephalosporins, generating more active β-lactam derivatives. Global prokaryotic genome analysis revealed the existence of a gene identical to estB in Pseudomonas aeruginosa strain PS1 suggesting a horizontal gene transfer event involving estB. To investigate the effect of ectopic expression of EstB in the periplasm of P. aeruginosa and several Enterobacteriaceae on antibiotic susceptibility levels, plasmid, pEstB, carrying a recombinant EstB fused with the signal peptide from Escherichia coli outer membrane protein A (OmpA) for periplasmic localization was constructed. The expression of EstB in the periplasm of P. aeruginosa and the Enterobacteriaceae: E. coli, Klebsiella pneumoniae, and Salmonella enterica serovar Typhi, increased susceptibility to carbapenems and cephalosporins. EstB reversed the imipenem resistance of P. aeruginosa ΔmexS and restored the changes in susceptibility to cephalosporins conferred by the downregulation of the outer membrane proteins, OmpK35 and OmpK36, in K. pneumoniae ΔramR-ompK36 to wild-type level. The introduction of EstB to the periplasmic space of Gram-negative bacteria can increase carbapenem and cephalosporin susceptibility.

重组酯酶 B 对耐亚胺培南铜绿假单胞菌的再敏感性和肠杆菌科细菌对头孢菌素的敏感性的恢复。
Sphingobium sp. SM42 Esterase B(ESTB)是一种具有双重功能的酶,它既能降解邻苯二甲酸二丁酯(DBP),又能催化头孢菌素二氢噻嗪环 C3-侧链中 C-S 键的裂解,生成活性更强的 β-内酰胺衍生物。全球原核生物基因组分析表明,铜绿假单胞菌菌株 PS1 中存在一个与 estB 相同的基因,这表明存在涉及 estB 的水平基因转移事件。为了研究在铜绿假单胞菌和几种肠杆菌科细菌的外周质粒中异位表达 EstB 对抗生素敏感性水平的影响,我们构建了质粒 pEstB,该质粒携带与大肠杆菌外膜蛋白 A(OmpA)信号肽融合的重组 EstB,用于外周质粒定位。在铜绿假单胞菌和肠杆菌科细菌(大肠杆菌、肺炎克雷伯菌和伤寒沙门氏菌)的外周质粒中表达 EstB 会增加对碳青霉烯类和头孢菌素的敏感性。EstB 逆转了铜绿假单胞菌 ΔmexS 的亚胺培南耐药性,并将肺炎克雷伯菌 ΔramR-ompK36 的外膜蛋白 OmpK35 和 OmpK36 下调到野生型水平所导致的对头孢菌素的敏感性变化恢复到野生型水平。将 EstB 引入革兰氏阴性菌的外质子空间可增加对碳青霉烯类和头孢菌素的敏感性。
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来源期刊
Letters in Applied Microbiology
Letters in Applied Microbiology 工程技术-生物工程与应用微生物
CiteScore
4.40
自引率
4.20%
发文量
225
审稿时长
3.3 months
期刊介绍: Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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