Optimizing Electroconductive PPy-PCL Scaffolds for Enhanced Tissue Engineering Performance

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-11-22 DOI:10.1002/jbm.b.35511

Ana M. Muñoz-González, Dianney Clavijo-Grimaldo, Sara Leal-Marin, Birgit Glasmacher

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引用次数: 0

Abstract

The integration of electrically conductive materials is a promising approach in tissue regeneration research. The study presented focuses on the creation of electroconductive scaffolds made from polypyrrole-polycaprolactone (PPy-PCL) using optimal processing parameters. Utilizing Box–Behnken response surface methodology for in situ chemical polymerization of PPy, the scaffolds exhibited a maximum conductivity of 2.542 mS/cm. Morphological examination via scanning electron microscopy (SEM) indicated uniform dispersion of PPy particles within PCL fibers. Fourier transform infrared spectroscopy (FTIR) and energy dispersive x-ray (EDX) analysis validated the composition of the scaffolds, while mechanical testing revealed that the optimized scaffolds exhibit superior tensile strength and Young's modulus compared to scaffolds comprised only of PCL. The hydrophilicity of the scaffolds was improved considerably, transitioning from initially hydrophobic to fully hydrophilic for the optimum scaffold, making it suitable for tissue engineering applications. Cell viability assays, including MTT with L929 fibroblasts and Alamar Blue with bone marrow mesenchymal stem cells (bmMSCs), reflected no cytotoxicity. They showed an increase in metabolic activity, suggesting the capability of the scaffolds to support cellular functions. In conclusion, the in situ synthesis of PPy in the PCL matrix by optimizing the fabrication parameters resulted in conductive scaffolds with promising structural and functional properties for tissue engineering.

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优化导电 PPy-PCL 支架，提高组织工程性能。

集成导电材料是组织再生研究中一种前景广阔的方法。本研究的重点是采用最佳加工参数，用聚吡咯-聚己内酯（PPy-PCL）制作导电支架。利用 Box-Behnken 响应面方法对 PPy 进行原位化学聚合，支架显示出 2.542 mS/cm 的最大电导率。扫描电子显微镜（SEM）的形态学检查表明，PPy 颗粒均匀地分散在 PCL 纤维中。傅立叶变换红外光谱（FTIR）和能量色散 X 射线（EDX）分析验证了支架的组成，而机械测试表明，与仅由 PCL 组成的支架相比，优化后的支架具有更高的拉伸强度和杨氏模量。支架的亲水性得到了显著改善，从最初的疏水性过渡到最佳支架的完全亲水性，使其适用于组织工程应用。细胞存活率检测（包括 L929 成纤维细胞的 MTT 检测和骨髓间充质干细胞（bmMSCs）的阿拉玛蓝检测）显示，支架没有细胞毒性。它们的新陈代谢活动有所增加，表明支架具有支持细胞功能的能力。总之，通过优化制造参数，在 PCL 基质中原位合成 PPy，可制成具有良好结构和功能特性的导电支架，用于组织工程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part B, Applied biomaterials 工程技术-材料科学：生物材料

CiteScore

7.50

自引率

2.90%

发文量

199

审稿时长

12 months

期刊介绍： Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.