Fabrication of norepinephrine and ε-polylysine engineered magnetic nanocomposites tailored for phosphopeptides analysis

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2024-11-17 DOI:10.1016/j.chroma.2024.465539

Yaming Zhao , Jinfeng Zhang , JunWei Yang , Jiutong Ma , Haipeng Zhang , Qiong Jia

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Abstract

Protein phosphorylation is a highly prevalent post-translational modification that holds a vital position in numerous physiological processes. Prior to mass spectrometry detection, the enrichment of phosphopeptides is critically significant due to their susceptibility to interference from abundant non-phosphopeptides. In this study, the magnetic nanocomposite (Fe₃O₄@NE@PL) was successfully synthesized and characterized. Fe₃O₄@NE@PL exhibited strong hydrophilicity, electrophilicity and intermolecular interactions through hydrogen bonds, enabling it to effectively enrich phosphopeptides with excellent sensitivity (0.4 fmol β-casein) and selectivity (β-casein:BSA=1:1000). In addition, Fe₃O₄@NE@PL was successfully applied to enrich phosphopeptides from complex real biological samples such as human serum and saliva, achieving up to 4 recycles with favorable stability and reusability. This study demonstrates that Fe₃O₄@NE@PL is a promising adsorbent for phosphopeptides enrichment in proteomics research, providing new ideas for the construction of magnetic enrichment materials.

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为磷酸肽分析量身定制的去甲肾上腺素和ε-聚赖氨酸工程磁性纳米复合材料的制备。

蛋白质磷酸化是一种非常普遍的翻译后修饰，在许多生理过程中都占有重要地位。在质谱检测之前，由于磷酸肽易受大量非磷酸肽的干扰，因此富集磷酸肽至关重要。本研究成功合成并表征了磁性纳米复合材料（Fe3O4@NE@PL）。Fe3O4@NE@PL具有很强的亲水性、亲电性和分子间氢键相互作用，能有效富集磷酸肽，且灵敏度（0.4 fmol β-酪蛋白）和选择性（β-酪蛋白:BSA=1:1000）极佳。此外，Fe3O4@NE@PL 还成功地应用于从人血清和唾液等复杂的真实生物样品中富集磷酸肽，最多可实现 4 次循环，具有良好的稳定性和重复使用性。该研究表明，Fe3O4@NE@PL 是蛋白质组学研究中一种很有前景的磷酸肽富集吸附剂，为磁性富集材料的构建提供了新思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.