Activatable Two-Photon-Excited Molecular Fluorescent Probes for Near-Infrared Biosensing and Bioimaging

ACS Applied Engineering Materials Pub Date : 2024-11-09 DOI:10.1021/acsaenm.4c0059810.1021/acsaenm.4c00598

Jingpi Gao, Ming Liu, Li Wu, Zhiyuan Tian*, Jian-Hong Tang* and Yujie Sun*,

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Abstract

Two-photon-excited fluorescence imaging (TPEFI) is a rapidly advancing technique for detecting analytes and visualizing biological conditions in real time. Compared to conventional one-photon excitation, TPEFI offers advantages, such as deeper tissue penetration, reduced photodamage, high sensitivity, and superior temporal resolution, making it highly suitable for in vivo imaging applications. The integration of biostimuli-responsive elements into two-photon fluorophores has enabled the development of bioactivatable two-photon-excited small molecules that are effective in near-infrared bioimaging for monitoring diverse biological processes and diseases. This review highlights recent progress (2019–2024) in the design and application of two-photon-excited near-infrared fluorescent probes specifically developed for activity-based bioimaging. We provide a succinct overview of both chemically and enzymatically activated probes by discussing their structural design principles, bioresponsive characteristics, two-photon absorption and emission properties, and their use in vitro and in vivo for monitoring specific biomedical conditions and diseases.

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用于近红外生物传感和生物成像的可激活双光子激发分子荧光探针

双光子激发荧光成像（TPEFI）是一种快速发展的实时检测分析物和观察生物状况的技术。与传统的单光子激发相比，双光子激发荧光成像具有穿透组织更深、减少光损伤、灵敏度高和时间分辨率高等优点，因此非常适合体内成像应用。将生物刺激响应元件集成到双光子荧光团中，开发出了可生物活化的双光子激发小分子，这些小分子在近红外生物成像中可有效监测各种生物过程和疾病。本综述重点介绍了专门为基于活性的生物成像而开发的双光子激发近红外荧光探针的设计和应用方面的最新进展（2019-2024 年）。我们通过讨论化学和酶激活探针的结构设计原理、生物响应特性、双光子吸收和发射特性，以及它们在体外和体内用于监测特定生物医学条件和疾病的情况，简明扼要地概述了这些探针。

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ACS Applied Engineering Materials 材料科学-

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期刊介绍： ACS Applied Engineering Materials is an international and interdisciplinary forum devoted to original research covering all aspects of engineered materials complementing the ACS Applied Materials portfolio. Papers that describe theory simulation modeling or machine learning assisted design of materials and that provide new insights into engineering applications are welcomed. The journal also considers experimental research that includes novel methods of preparing characterizing and evaluating new materials designed for timely applications. With its focus on innovative applications ACS Applied Engineering Materials also complements and expands the scope of existing ACS publications that focus on materials science discovery including Biomacromolecules Chemistry of Materials Crystal Growth & Design Industrial & Engineering Chemistry Research Inorganic Chemistry Langmuir and Macromolecules.The scope of ACS Applied Engineering Materials includes high quality research of an applied nature that integrates knowledge in materials science engineering physics mechanics and chemistry.