miR394 and LCR cooperate with TPL to regulate AM initiation

IF 14.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2024-11-23 DOI:10.1038/s41467-024-54494-6

Liya Liu, Binbin Hu, Siying Guo, Zhihui Xue, Tao Wang, Cui Zhang

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Abstract

Plant architecture is a main determinate of crop yield, and lateral branching significantly influences the number of inflorescences and seeds. The mechanism of axillary bud initiation remains unclear. This work aimed to examine how miRNAs regulate axillary bud initiation. By constructing a small RNA library and screening a mutant population, we revealed the initiation of axillary buds is specifically induced by miR394 and repressed by its target, LEAF CURLING RESPONSIVENESS (LCR). Using promoter-driven fluorescent tags and in situ hybridization, we showed that miR394 is localized in the center of the leaf axil where AMs are initiated. Through molecular and genetic research, we revealed that miR394/LCR may regulate REVOLUTA (REV) and SHOOT MERISTEMLESS (STM) to establish the axillary meristem. Immunoprecipitation-mass spectrometry studies revealed that LCR, as an F-box protein, may interact with TOPLESS (TPL) proteins and participate in ubiquitinated protein degradation. Our results reveal an important mechanism by which the miR394-regulated LCR accelerates the degradation of TPL to precisely modulate axillary bud initiation.

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miR394 和 LCR 与 TPL 合作调控 AM 的启动

植株结构是作物产量的主要决定因素，而侧枝对花序和种子的数量有很大影响。腋芽萌发的机制尚不清楚。这项工作旨在研究 miRNA 如何调控腋芽的萌发。通过构建小 RNA 文库和筛选突变体群体，我们发现腋芽的萌发受 miR394 的特异性诱导，而受其靶标 LEAF CURLING RESPONSIVENESS (LCR) 的抑制。利用启动子驱动的荧光标签和原位杂交技术，我们发现 miR394 定位于叶腋中心，而 AM 就在叶腋中心启动。通过分子和遗传研究，我们发现miR394/LCR可能调控REVOLUTA（REV）和SHOOT MERISTEMLESS（STM）以建立腋生分生组织。免疫沉淀-质谱研究发现，LCR作为一种F-盒蛋白，可能与TOPLESS（TPL）蛋白相互作用，并参与泛素化蛋白降解。我们的研究结果揭示了 miR394 调控的 LCR 加速 TPL 降解以精确调节腋芽萌发的重要机制。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.