Investigation of sex determination in starry flounder ( Platichthys stellatus) reveals sex chromosome evolution in Pleuronectiformes and identifies a sex-specific marker.

IF 4 1区生物学 Q1 ZOOLOGY

Zoological Research Pub Date : 2024-11-18 DOI:10.24272/j.issn.2095-8137.2024.078

Wei-Jing Li, Yu-Man Zhang, Shuo Li, Yu-Yan Liu, Chen Li, Sheng-Lei Han, Kai-Qiang Liu, Lucas Benites Doretto, Bing-Hua Liu, Hai-Jun Huang, Zhi-Long Sun, Qian Wang, Hong-Yan Wang, Chang-Wei Shao

{"title":"Investigation of sex determination in starry flounder ( Platichthys stellatus) reveals sex chromosome evolution in Pleuronectiformes and identifies a sex-specific marker.","authors":"Wei-Jing Li, Yu-Man Zhang, Shuo Li, Yu-Yan Liu, Chen Li, Sheng-Lei Han, Kai-Qiang Liu, Lucas Benites Doretto, Bing-Hua Liu, Hai-Jun Huang, Zhi-Long Sun, Qian Wang, Hong-Yan Wang, Chang-Wei Shao","doi":"10.24272/j.issn.2095-8137.2024.078","DOIUrl":null,"url":null,"abstract":"The identification of sex chromosomes is fundamental for exploring the mechanism and evolution of sex determination. Platichthys stellatus, a species exhibiting clear sexual dimorphism and homomorphic chromosome pairs, has received limited research concerning its sex determination mechanisms. Clarifying the sex chromosome of P. stellatus will enhance our understanding of sex chromosome evolution in Pleuronectiformes. This study employed whole-genome resequencing to investigate the sex chromosome and sex determination system in P. stellatus. Notably, Chr23 was identified as the sex chromosome in P. stellatus, with the sex-determining region (SDR) occupying 48.1% of the chromosome and featuring an XX/XY system. Sex chromosome turnover was observed within Pleuronectiformes, with P. stellatus, Verasper variegatus, and Hippoglossus hippoglossus sharing a common ancestral karyotype. No inversions were detected within the SDR of P. stellatus, although chromosomal rearrangements between sex chromosomes and autosomes were identified. Additionally, a sex-specific marker for P. stellatus was ascertained, enabling genetic sex identification, with significant implications for improving breeding programs and aquaculture practices.","PeriodicalId":48636,"journal":{"name":"Zoological Research","volume":"45 6","pages":"1347-1356"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoological Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.24272/j.issn.2095-8137.2024.078","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ZOOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The identification of sex chromosomes is fundamental for exploring the mechanism and evolution of sex determination. Platichthys stellatus, a species exhibiting clear sexual dimorphism and homomorphic chromosome pairs, has received limited research concerning its sex determination mechanisms. Clarifying the sex chromosome of P. stellatus will enhance our understanding of sex chromosome evolution in Pleuronectiformes. This study employed whole-genome resequencing to investigate the sex chromosome and sex determination system in P. stellatus. Notably, Chr23 was identified as the sex chromosome in P. stellatus, with the sex-determining region (SDR) occupying 48.1% of the chromosome and featuring an XX/XY system. Sex chromosome turnover was observed within Pleuronectiformes, with P. stellatus, Verasper variegatus, and Hippoglossus hippoglossus sharing a common ancestral karyotype. No inversions were detected within the SDR of P. stellatus, although chromosomal rearrangements between sex chromosomes and autosomes were identified. Additionally, a sex-specific marker for P. stellatus was ascertained, enabling genetic sex identification, with significant implications for improving breeding programs and aquaculture practices.

查看原文本刊更多论文

星鲽的性别决定研究揭示了 Pleuronectiformes 的性染色体进化，并确定了一个性别特异性标记。

性染色体的鉴定是探索性别决定机制和进化的基础。栉水母（Platichthys stellatus）表现出明显的性二型和同形染色体对，但对其性别决定机制的研究却很有限。阐明栉水母的性染色体将有助于我们了解栉水母的性染色体进化。本研究采用全基因组重测序技术研究了星尾栉水母的性染色体和性别决定系统。值得注意的是，Chr23被鉴定为尾柱虫的性染色体，其性别决定区（SDR）占染色体的48.1%，具有XX/XY系统。在 Pleuronectiformes 中观察到了性染色体的更替，P. stellatus、Verasper variegatus 和 Hippoglossus 具有共同的祖先核型。虽然发现了性染色体与常染色体之间的染色体重排，但在星尾蜥的性染色体重排中未发现倒位。此外，还确定了黄颡鱼的性别特异性标记，实现了遗传性别鉴定，对改进育种计划和水产养殖实践具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Zoological Research Medicine-General Medicine

CiteScore

7.60

自引率

10.20%

发文量

1937

审稿时长

8 weeks

期刊介绍： Established in 1980, Zoological Research (ZR) is a bimonthly publication produced by Kunming Institute of Zoology, the Chinese Academy of Sciences, and the China Zoological Society. It publishes peer-reviewed original research article/review/report/note/letter to the editor/editorial in English on Primates and Animal Models, Conservation and Utilization of Animal Resources, and Animal Diversity and Evolution.