{"title":"Introgression and Mapping of a Novel Bacterial Blight Resistance Gene <i>xa49(t)</i> from <i>Oryza rufipogon</i> acc. CR100098A into <i>O. sativa</i>.","authors":"Sukhpreet Kaur Bhatia, Yogesh Vikal, Pavneet Kaur, Gurmanpuneet Singh Dhillon, Gurwinder Kaur, Kumari Neelam, Palvi Malik, Jagjeet Singh Lore, Renu Khanna, Kuldeep Singh","doi":"10.1094/PHYTO-02-24-0061-R","DOIUrl":null,"url":null,"abstract":"<p><p>Bacterial blight (BB) caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> is one of the epidemic diseases in rice. Rapid changes in the pathogenicity of the <i>X. oryzae</i> pv. <i>oryzae</i> pathogen demand the identification and characterization of novel BB resistance genes. Here, we report the transfer and mapping of a new BB resistance gene from <i>Oryza rufipogon</i> acc. CR100098A. Inheritance studies on the BC<sub>2</sub>F<sub>2</sub> population, BC<sub>2</sub>F<sub>3</sub> progenies, and backcross-derived recombinant inbred lines derived from a cross between Pusa44/<i>O. rufipogon</i> acc. CR100098A//2<sup>*</sup>PR114 showed that a single recessive gene confers resistance in <i>O. rufipogon</i> acc. CR100098A. Bulked segregant analysis using 203 simple sequence repeat (SSR) markers localized the BB resistance gene on chromosome 11 bracketed between two SSR markers, RM27235 and RM2136. Using PR114 and <i>O. rufipogon</i> acc. CR100098A genotyping by sequencing data, 86 KASP markers within the bracketed region were designed and tested for bulked segregant analysis. Only five KASP markers showed polymorphism between parents, and three were associated with the target gene. Seventy-seven new SSR markers were designed from the same interval. A total of 33 polymorphic markers were analyzed on the whole population and mapped the BB gene in an interval of 2.8 cM flanked by SSR markers PAU11_65 and PAU11_44 within a physical distance of 376.3 kb. The BB resistance gene mapped in this study is putatively new and designated as <i>xa49(t)</i>. Fourteen putative candidate genes were identified within the <i>xa49(t)</i> region having a role in biotic stress resistance. The linked markers to the <i>xa49(t)</i> gene were validated in other rice cultivars for its successful deployment in BB resistance breeding.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":"2412-2420"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-02-24-0061-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae is one of the epidemic diseases in rice. Rapid changes in the pathogenicity of the X. oryzae pv. oryzae pathogen demand the identification and characterization of novel BB resistance genes. Here, we report the transfer and mapping of a new BB resistance gene from Oryza rufipogon acc. CR100098A. Inheritance studies on the BC2F2 population, BC2F3 progenies, and backcross-derived recombinant inbred lines derived from a cross between Pusa44/O. rufipogon acc. CR100098A//2*PR114 showed that a single recessive gene confers resistance in O. rufipogon acc. CR100098A. Bulked segregant analysis using 203 simple sequence repeat (SSR) markers localized the BB resistance gene on chromosome 11 bracketed between two SSR markers, RM27235 and RM2136. Using PR114 and O. rufipogon acc. CR100098A genotyping by sequencing data, 86 KASP markers within the bracketed region were designed and tested for bulked segregant analysis. Only five KASP markers showed polymorphism between parents, and three were associated with the target gene. Seventy-seven new SSR markers were designed from the same interval. A total of 33 polymorphic markers were analyzed on the whole population and mapped the BB gene in an interval of 2.8 cM flanked by SSR markers PAU11_65 and PAU11_44 within a physical distance of 376.3 kb. The BB resistance gene mapped in this study is putatively new and designated as xa49(t). Fourteen putative candidate genes were identified within the xa49(t) region having a role in biotic stress resistance. The linked markers to the xa49(t) gene were validated in other rice cultivars for its successful deployment in BB resistance breeding.
期刊介绍：
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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