Brief guide to immunostaining.

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2024-11-19 DOI:10.1016/j.mocell.2024.100157

Gyutae Park, Sieun S Kim, Jiwon Shim, Seung-Jae V Lee

引用次数: 0

Abstract

Immunostaining is an essential biological technique that determines the localization and level of target antigen molecules, using antibodies within cells or tissues. Here we present a brief guide to immunostaining, including the principles, methods and different types of immunostaining. This manuscript will also provide common challenges and optimization strategies. This work will be useful for researchers with basic knowledge in immunostaining.

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免疫染色简要指南。

免疫染色是一种重要的生物学技术，它利用细胞或组织中的抗体来确定目标抗原分子的定位和水平。在此，我们将简要介绍免疫染色的原理、方法和不同类型的免疫染色。本手稿还将提供常见挑战和优化策略。这项工作将对具备免疫染色基础知识的研究人员有所帮助。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.