Chemical constituents from the culture of Biscogniauxia capnodes SWUF15-40 fungus and the inhibitory activity against cancer cell proliferation and nitric oxide production.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2024-11-22 DOI:10.1080/14786419.2024.2432611

Audomsak Churat, Jaursup Boonmak, Wiyada Mongkolthanaruk, Pairot Moontragoon, Thanaset Senawong, Nuttika Suwannasai, Sirirath McCloskey

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Abstract

The chemical diversity from Biscogniauxia capnodes SWUF15-40 fungus, cultivated in yeast-malt extract medium was studied. The choice of medium was inspired by the OSMACs strategy, which had previously been cultivated in PDB medium. This resulted in an array of bioactive compounds being discovered. The IR, NMR, MS, and XRD data, together with the calculated ¹³C NMR chemical shifts and ECD spectra data were thoroughly analysed, which led to the determination of two new isopimarane (1 and 2), and three guaiane (3-5) derivatives, together with four known compounds (6-9). Cyclic pentapeptide (9) showed inhibitory activity against cancer cells (HeLa, HT-29, PC-3, and A549) proliferation and nitric oxide (NO) production in LPS-stimulated RAW264.7 cells with IC₅₀ values in the ranges of 11.38 - 17.87 µM, and 58.88 µg/mL, respectively. The results demonstrated that manipulating growth conditions can increase the possibility of discovering many more bioactive compounds in fungi.

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Biscogniauxia capnodes SWUF15-40 真菌培养物中的化学成分及其对癌细胞增殖和一氧化氮生成的抑制活性。

研究了在酵母麦芽提取物培养基中培养的 Biscogniauxia capnodes SWUF15-40 真菌的化学多样性。培养基的选择受到 OSMACs 战略的启发，OSMACs 以前曾在 PDB 培养基中培养。结果发现了一系列生物活性化合物。对红外光谱、核磁共振、质谱和 XRD 数据，以及计算得出的 13C 核磁共振化学位移和 ECD 光谱数据进行了深入分析，从而确定了两种新的异丙马兰（1 和 2）和三种愈创木烷（3-5）衍生物，以及四种已知化合物（6-9）。环五肽（9）对癌细胞（HeLa、HT-29、PC-3 和 A549）的增殖和 LPS 刺激下 RAW264.7 细胞产生一氧化氮（NO）具有抑制活性，IC50 值分别为 11.38 - 17.87 µM 和 58.88 µg/mL。研究结果表明，操纵生长条件可以增加发现真菌中更多生物活性化合物的可能性。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.