Regeneration of Chronic Alveolar Vertical Defects Using a Micro Dosage of rhBMP-2. An Experimental In Vivo Study.

IF 4.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Oral Implants Research Pub Date : 2024-11-22 DOI:10.1111/clr.14379

Istvan A Urban, Sándor Farkasdi, Dieter D Bosshardt, Mauricio G Araujo, Andrea Ravidà, Kathrin Becker, Robert Kerberger, Hom-Lay Wang, Ulf M E Wikesjö, Gabor Varga, Muhammad H A Saleh

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引用次数: 0

Abstract

Objective: The objective of this study is to compare the effect of the location of recombinant human bone morphogenetic protein 2 (rhBMP-2) from the native bone and the periosteum for vertical alveolar bone augmentation.

Materials and methods: Mandibular, chronic, standardized, bilateral, and vertical defects in 12 beagle dogs were evaluated using four modalities: a xenograft alone (XENO; n = 6); rhBMP-2 alone (BMP; n = 6); a technique with rhBMP-2 close to the host bone covered by xenograft (SAN; n = 6); and a technique with rhBMP-2 close to the flap on top of the xenograft (LAS; n = 6). After 8 weeks, a series of in vivo inspections, fluorescence microscopy, histologic and histomorphometric evaluations, and micro-CT analyses.

Results: After 8 weeks of healing, new bone formation correlated with proximity of rhBMP to the perforated membrane with BMP and LAS (p = 0.024). The highest total bone volume was found in the LAS group (45.1% ± 13.3%), followed by the SAN group (35.2% ± 6.7%), BMP group (33.1% ± 11.8%), followed by the XENO group (23.1% ± 6.5%). The SAN group demonstrated frequent seroma formation. Blood vessel formation was more pronounced in the LAS + rhBMP group, with a significant increase of 27.1% compared to the XENO group (p = 0.02). Micro-CT revealed a strong trend for higher bone volume in the BMP group (34.7%) compared to the XENO group (13.6%) (p = 0.06). Only rhBMP-2 groups demonstrated bone formation above the perforated membrane.

Conclusion: The location of rhBMP-2 in relation to the biomaterial and periosteum influenced the effectiveness of vertical bone regeneration.

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使用微量 rhBMP-2 使慢性牙槽垂直缺损再生。体内实验研究。

研究目的本研究的目的是比较重组人骨形态发生蛋白 2（rhBMP-2）从原生骨和骨膜的位置用于垂直牙槽骨增量的效果：对12只猎犬的下颌骨、慢性、标准化、双侧和垂直缺损进行了评估，采用了四种方法：单独使用异种移植（XENO；n = 6）；单独使用rhBMP-2（BMP；n = 6）；rhBMP-2接近异种移植覆盖的宿主骨的技术（SAN；n = 6）；以及rhBMP-2接近异种移植上的皮瓣的技术（LAS；n = 6）。8 周后，进行一系列体内检查、荧光显微镜检查、组织学和组织形态计量学评估以及显微 CT 分析：结果：愈合 8 周后，新骨形成与 rhBMP 与 BMP 和 LAS 的穿孔膜距离相关（p = 0.024）。骨总量最高的是 LAS 组（45.1% ± 13.3%），其次是 SAN 组（35.2% ± 6.7%）、BMP 组（33.1% ± 11.8%）和 XENO 组（23.1% ± 6.5%）。SAN 组经常出现血清肿形成。LAS + rhBMP 组的血管形成更为明显，比 XENO 组显著增加 27.1%（p = 0.02）。显微 CT 显示，与 XENO 组（13.6%）相比，BMP 组（34.7%）的骨量有明显增加的趋势（p = 0.06）。只有 rhBMP-2 组显示穿孔膜上方有骨形成：rhBMP-2与生物材料和骨膜的位置关系影响了垂直骨再生的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Oral Implants Research 医学-工程：生物医学

CiteScore

7.70

自引率

11.60%

发文量

149

审稿时长

3 months

期刊介绍： Clinical Oral Implants Research conveys scientific progress in the field of implant dentistry and its related areas to clinicians, teachers and researchers concerned with the application of this information for the benefit of patients in need of oral implants. The journal addresses itself to clinicians, general practitioners, periodontists, oral and maxillofacial surgeons and prosthodontists, as well as to teachers, academicians and scholars involved in the education of professionals and in the scientific promotion of the field of implant dentistry.