Carboxymethylation of β-Glucan from Pythium insidiosum: Structural characterization and preliminary adsorption evaluation of DON and T2 toxin.

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2024-11-17 DOI:10.1016/j.carres.2024.109323

Juliana S M Tondolo, Gilson Zeni, Guilherme L Sassaki, Janio M Santurio, Erico S Loreto

{"title":"Carboxymethylation of β-Glucan from Pythium insidiosum: Structural characterization and preliminary adsorption evaluation of DON and T2 toxin.","authors":"Juliana S M Tondolo, Gilson Zeni, Guilherme L Sassaki, Janio M Santurio, Erico S Loreto","doi":"10.1016/j.carres.2024.109323","DOIUrl":null,"url":null,"abstract":"This study aimed to evaluate the impact of carboxymethylation on the structural and functional properties of β-glucan derived from the pathogenic oomycete Pythium insidiosum. β-Glucan was extracted and subjected to carboxymethylation (CM-glucan), with structural changes analyzed using 13C and 1H NMR spectroscopy. The modified β-glucan's ability to adsorb mycotoxins, specifically deoxynivalenol (DON) and T2 toxin, was assessed through in vitro adsorption assays. Results demonstrated that the adsorption of DON by CM-glucan increased from 0 % to 59.11 %, corresponding to the adsorption of approximately 1.18 μg of DON from the initial concentration (2 μg/mL). Similarly, the adsorption of T2 toxin increased slightly from 0 % to 4.54 %, corresponding to 0.09 μg of T2 toxin adsorbed from the initial concentration (2 μg/mL). These findings underscore the potential of chemical modifications to enhance the functional properties of natural polysaccharides, suggesting future applications in mycotoxin adsorption and other biological properties across various areas.","PeriodicalId":9415,"journal":{"name":"Carbohydrate Research","volume":"547 ","pages":"109323"},"PeriodicalIF":2.4000,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.carres.2024.109323","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study aimed to evaluate the impact of carboxymethylation on the structural and functional properties of β-glucan derived from the pathogenic oomycete Pythium insidiosum. β-Glucan was extracted and subjected to carboxymethylation (CM-glucan), with structural changes analyzed using ¹³C and ¹H NMR spectroscopy. The modified β-glucan's ability to adsorb mycotoxins, specifically deoxynivalenol (DON) and T2 toxin, was assessed through in vitro adsorption assays. Results demonstrated that the adsorption of DON by CM-glucan increased from 0 % to 59.11 %, corresponding to the adsorption of approximately 1.18 μg of DON from the initial concentration (2 μg/mL). Similarly, the adsorption of T2 toxin increased slightly from 0 % to 4.54 %, corresponding to 0.09 μg of T2 toxin adsorbed from the initial concentration (2 μg/mL). These findings underscore the potential of chemical modifications to enhance the functional properties of natural polysaccharides, suggesting future applications in mycotoxin adsorption and other biological properties across various areas.

查看原文本刊更多论文

内生 Pythium β-葡聚糖的羧甲基化：DON 和 T2 毒素的结构特征和初步吸附评估。

本研究旨在评估羧甲基化对来自致病性卵菌内生毕赤霉的β-葡聚糖的结构和功能特性的影响。通过体外吸附试验评估了改良后的β-葡聚糖吸附霉菌毒素（特别是脱氧雪腐镰刀菌烯醇（DON）和 T2 毒素）的能力。结果表明，CM-葡聚糖对 DON 的吸附率从 0 % 增加到 59.11 %，相当于从初始浓度（2 μg/mL）中吸附了约 1.18 μg DON。同样，T2 毒素的吸附率也略有增加，从 0% 增加到 4.54%，相当于从初始浓度（2 微克/毫升）中吸附了 0.09 微克 T2 毒素。这些发现凸显了化学修饰在增强天然多糖功能特性方面的潜力，预示着化学修饰在霉菌毒素吸附和其他生物特性方面的应用前景广阔。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".