Plastid translocon recycling in dinoflagellates demonstrates the portability of complex plastids between hosts.

IF 8.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Current Biology Pub Date : 2024-12-02 Epub Date: 2024-11-20 DOI:10.1016/j.cub.2024.10.034
William H Lewis, Giulia Paris, Girish Beedessee, Ludek Kořený, Victor Flores, Tom Dendooven, Benoit Gallet, Daniel P Yee, Simon Lam, Johan Decelle, Ben F Luisi, Ross F Waller
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引用次数: 0

Abstract

The plastids of photosynthetic organisms on land are predominantly "primary plastids," derived from an ancient endosymbiosis of a cyanobacterium. Conversely, the plastids of marine photosynthetic organisms were mostly gained through subsequent endosymbioses of photosynthetic eukaryotes generating so-called "complex plastids." The plastids of the major eukaryotic lineages-cryptophytes, haptophytes, ochrophytes, dinoflagellates, and apicomplexans-were posited to derive from a single secondary endosymbiosis of a red alga in the "chromalveloate" hypothesis. Subsequent phylogenetic resolution of eukaryotes has shown that separate events of plastid acquisition must have occurred to account for this distribution of plastids. However, the number of such events and the donor organisms for the new plastid endosymbioses are still not resolved. A perceived bottleneck of endosymbiotic plastid gain is the development of protein targeting from the hosts into the new plastids, and this supposition has often driven hypotheses toward minimizing the number of plastid-gain events to explain plastid distribution in eukaryotes. But how plastid-protein-targeting is established for new endosymbionts is often unclear, which makes it difficult to assess the likelihood of plastid transfers between lineages. Here, we show that Kareniaceae dinoflagellates, which possess complex plastids known to be derived from haptophytes, acquired all the necessary protein import machinery from these haptophytes. Furthermore, cryo-electron tomography revealed that no additional membranes were added to the Kareniaceae complex plastid during serial endosymbiosis, suggesting that the haptophyte-derived import processes were sufficient. Our analyses suggest that complex red plastids are preadapted for horizontal transmission, potentially explaining their widespread distribution in algal diversity.

甲藻的质体转座子再循环证明了复杂质体在宿主之间的可移植性。
陆地上光合生物的质体主要是 "初级质体",来自蓝藻的古老内共生。相反,海洋光合生物的质体大多是通过随后的光合真核生物内共生获得的,产生了所谓的 "复合质体"。真核生物的主要类群--隐藻、隐球藻、水藻、甲藻和无柄类--的质体被假定为来自于 "染色单胞藻 "假说中的红藻的一次次级内共生。随后的真核生物系统发育分析表明,必须发生过不同的质体获得事件,才能解释质体的这种分布。然而,这类事件的数量以及新的质体内共生的供体生物仍然没有得到解决。人们认为内共生质体获得的一个瓶颈是蛋白质从宿主到新质体的靶向发展,而这一假设常常促使人们尽量减少质体获得事件的数量,以解释真核生物中质体的分布。但是,如何为新的内共生体建立质体蛋白靶向往往并不清楚,这就很难评估质体在不同种系之间转移的可能性。在这里,我们展示了卡伦鞭毛虫科甲藻,它们拥有已知来源于合子的复杂质体,并从这些合子获得了所有必要的蛋白质导入机制。此外,低温电子断层扫描显示,在连续内共生过程中,卡伦藻科复合质体没有添加额外的膜,这表明来自合子的导入过程是足够的。我们的分析表明,复合红色质体预先适应了水平传播,这可能是它们在藻类多样性中广泛分布的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Biology
Current Biology 生物-生化与分子生物学
CiteScore
11.80
自引率
2.20%
发文量
869
审稿时长
46 days
期刊介绍: Current Biology is a comprehensive journal that showcases original research in various disciplines of biology. It provides a platform for scientists to disseminate their groundbreaking findings and promotes interdisciplinary communication. The journal publishes articles of general interest, encompassing diverse fields of biology. Moreover, it offers accessible editorial pieces that are specifically designed to enlighten non-specialist readers.
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