Androgens suppress the sialyltransferases ST3GAL1 and ST3GAL4 and modulate mucin 10 glycosylation in the submandibular gland, related to sex differences in commensal microbiota composition in mice.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-11-21 DOI:10.1093/bbb/zbae175

Mana Deminami, Miku Hashimoto, Hiroki Takahashi, Naoki Harada, Yukari Minami, Tomoya Kitakaze, Wataru Masuda, Shigeo Takenaka, Hiroshi Inui, Ryoichi Yamaji

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引用次数: 0

Abstract

Sex differences exist in the commensal microbiota that impact on multiple physiological processes in the host. Here, we examined the mechanism by which the sex differences are formed. In addition to the epithelial ductal cell, the acinar cell mass in the submandibular gland was associated with androgen-androgen receptor (AR) signaling. There was a sex difference in the formation of submandibular mucin 10 (MUC10) in SDS-PAGE. Neuraminidase treatment, which hydrolyzes terminal sialic acid, influenced the mobility shift of MUC10. Androgen-AR signaling negatively regulated St3 β-galactoside α-2,3-sialyltransferase 1 (St3gal1) and St3gal4 in the submandibular gland. There was a trend and significant sex differences in α-diversity (Shannon, p=0.09) and β-diversity (unweighted UniFrac) in oral microbiota composition, respectively. Some female-preferring bacteria including Akkermansia muciniphila can assimilate mucin by degrading terminal sialic acids. Our results indicate that androgen-AR signaling suppresses ST3GAL1 and ST3GAL4, which can influence sex differences in commensal microbiota composition.

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雄激素抑制硅氨酰基转移酶ST3GAL1和ST3GAL4并调节颌下腺粘蛋白10的糖基化，这与小鼠共生微生物群组成的性别差异有关。

共生微生物群存在性别差异，对宿主的多种生理过程产生影响。在这里，我们研究了性别差异的形成机制。除了上皮导管细胞外，颌下腺中的针状细胞团也与雄激素-雄激素受体（AR）信号传导有关。在 SDS-PAGE 中，颌下腺粘蛋白 10（MUC10）的形成存在性别差异。神经氨酸酶处理可水解末端序列酸，从而影响MUC10的迁移率变化。雄激素-AR信号负调控颌下腺中的St3 β-半乳糖苷 α-2,3-酰基转移酶1（St3gal1）和St3gal4。口腔微生物群组成中的α多样性（香农，p=0.09）和β多样性（非加权UniFrac）分别存在趋势和显著的性别差异。包括 Akkermansia muciniphila 在内的一些雌性偏好细菌可以通过降解末端硅酸来同化粘蛋白。我们的研究结果表明，雄激素-AR 信号抑制 ST3GAL1 和 ST3GAL4，从而影响共生微生物群组成的性别差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).