Increased antibiotic resistance gene abundance linked to intensive bacterial competition in the phyllosphere across an elevational gradient.

IF 3.6 4区 生物学 Q2 ENVIRONMENTAL SCIENCES
Yihui Ding, Rui-Ao Ma, Ran Zhang, Hongwei Zhang, Jian Zhang, Shaopeng Li, Si-Yu Zhang
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引用次数: 0

Abstract

Antibiotic resistance genes (ARGs) are ancient and widespread in natural habitats, providing survival advantages for microbiomes under challenging conditions. In mountain ecosystems, phyllosphere bacterial communities face multiple stress conditions, and the elevational gradients of mountains represent crucial environmental gradients for studying biodiversity distribution patterns. However, the distribution patterns of ARGs in the phyllosphere along elevational gradients, and their correlation with bacterial community structures, remain poorly understood. Here, we applied metagenomic analyses to investigate the abundance and diversity of ARGs in 88 phyllosphere samples collected from Mount Tianmu, a national natural reserve. Our results showed that the abundance of ARGs in the phyllosphere increased along elevational gradients and was dominated by multidrug resistance and efflux pumps. The composition of bacterial communities, rather than plant traits or abiotic factors, significantly affected ARG abundance. Moreover, increased ARG abundance was correlated with greater phylogenetic overdispersion and a greater proportion of negative associations in the bacterial co-occurrence networks, suggesting that bacterial competition primarily shapes phyllosphere resistomes. These findings constitute a major advance in the biodiversity of phyllosphere resistomes along elevations, emphasizing the significant impact of bacterial community structure and assembly on ARG distribution, and are essential for understanding the emergence of ARGs.

抗生素抗性基因丰度的增加与跨海拔梯度植物圈中激烈的细菌竞争有关。
抗生素耐药基因(ARGs)在自然栖息地中古老而广泛,为微生物群落在挑战性条件下的生存提供了优势。在山区生态系统中,叶球细菌群落面临多种压力条件,而山区的海拔梯度是研究生物多样性分布模式的重要环境梯度。然而,人们对ARGs沿海拔梯度在植物圈中的分布模式及其与细菌群落结构的相关性仍然知之甚少。在此,我们应用元基因组学分析方法研究了从国家级自然保护区天目山采集的88份植物圈样本中ARGs的丰度和多样性。结果表明,ARGs在植物叶球中的丰度随着海拔梯度的增加而增加,并且以多药耐药性和外排泵为主。细菌群落的组成而非植物性状或非生物因素对ARG丰度有显著影响。此外,ARG丰度的增加与细菌共生网络中更大的系统发育过度分散性和更大的负相关比例相关,这表明细菌竞争主要塑造了叶球抗性组。这些发现是海拔高度植物叶球抗逆群落生物多样性方面的重大进展,强调了细菌群落结构和组装对抗逆群落分布的重要影响,对理解抗逆群落的出现至关重要。
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来源期刊
Environmental Microbiology Reports
Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY
CiteScore
6.00
自引率
3.00%
发文量
91
审稿时长
3.0 months
期刊介绍: The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.
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