Engineering Thermoresponsive Enzyme-Polymer Conjugates via Glycan-Selective In Situ Polymerization for Recyclable Homogeneous Biocatalysis.

Abstract

Enzymes are crucial for various technological applications, but their inherent instability and short lifespan pose challenges. This study presents facile immobilized enzyme technology with the development of thermoresponsive enzyme-polymer conjugates (EPCs), using glucose oxidase (GOx) as a model enzyme, to address these limitations. By conjugating heteropolymers to the glycan moieties of GOx through a precise in situ polymerization process, we could modulate the lower critical solution temperature of the EPCs, enhancing enzyme performance without compromising its active site. The EPCs demonstrate a switchable behavior that facilitates efficient homogeneous catalysis and easy heterogeneous separation, reducing costs and environmental impact in industrial applications. Our strategy presents a versatile platform for creating efficient biocatalysts with tunable properties, marking a step forward in sustainable and cost-effective bioprocessing.