{"title":"Synthesis of a fluorescent probe based on rhodol's highly selective recognition of H<sub>2</sub>S and its application in cells.","authors":"Jiefeng Tang, Xiangjun Chen, Zhenzhen Wang, Shuntao Zhang, Juan Wang, Chunru Cheng","doi":"10.1039/d4ay01643b","DOIUrl":null,"url":null,"abstract":"<p><p>A new fluorescent probe for detecting hydrogen sulfide (H<sub>2</sub>S) was developed through the thiolation reaction of 2-chloro-1,4-naphthoquinone. Rhodol was employed as the fluorophore, while 2-chloro-1,4-naphthoquinone acted as the reactive group for H<sub>2</sub>S. The probe remains non-fluorescent under sunlight but emits a strong fluorescence upon reaction with H<sub>2</sub>S, accompanied by a visible color change in the solution. This selective H<sub>2</sub>S probe features rapid detection (under 1 minute), high photostability, and a very low detection limit (LOD = 44.3 nM), well below the levels that trigger physiological responses. The probe's mechanism was confirmed through <sup>1</sup>H-NMR, HR-MS, and DFT analysis. With low cytotoxicity and high biocompatibility, the probe has been effectively applied for fluorescent bioimaging of H<sub>2</sub>S in living cells.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ay01643b","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
A new fluorescent probe for detecting hydrogen sulfide (H2S) was developed through the thiolation reaction of 2-chloro-1,4-naphthoquinone. Rhodol was employed as the fluorophore, while 2-chloro-1,4-naphthoquinone acted as the reactive group for H2S. The probe remains non-fluorescent under sunlight but emits a strong fluorescence upon reaction with H2S, accompanied by a visible color change in the solution. This selective H2S probe features rapid detection (under 1 minute), high photostability, and a very low detection limit (LOD = 44.3 nM), well below the levels that trigger physiological responses. The probe's mechanism was confirmed through 1H-NMR, HR-MS, and DFT analysis. With low cytotoxicity and high biocompatibility, the probe has been effectively applied for fluorescent bioimaging of H2S in living cells.
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