Re-localization of a repeat-containing fungal effector by apoplastic protein Chitinase-like 1 blocks its toxicity

IF 14.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2024-11-22 DOI:10.1038/s41467-024-54470-0

Hanqiao Liu, Wenshu Zhang, Qinqfei He, Reyila Aikemu, Huijuan Xu, Zhan Guo, Lu Wang, Weixi Li, Guilin Wang, Xinyu Wang, Wangzhen Guo

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Abstract

A fungal effector that is toxic to plant cells was identified in Verticillium dahliae. The effector contains a non-canonical Common in several Fungal Extracellular Membrane proteins (CFEM) domain, a tandem repeat region consisting of four 14-amino acid repeats rich in proline, and a C-terminal region, thus is designated V. dahliae tetrapeptide repeat protein (VdTRP). The membrane targeting of VdTRP is vital for its cell toxicity. CFEM mediates the membrane targeting and the tandem repeat region exerts the toxic function upon cell membrane. The chitinase-like 1 (CTL1), an essential apoplastic protein of cotton, can redirect VdTRP from cell membrane to apoplast. Transgenic cotton overexpressing CTL1 greatly enhances cotton resistance to V. dahliae without affecting cotton growth and development, implicating its potential application in breeding cotton with high wilt resistance. Our data demonstrates that genetic manipulation of effector target constitutes potential strategy for improving crop resistance to fungal pathogens.

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细胞外蛋白几丁质酶样 1 对含重复的真菌效应子的再定位可阻止其毒性

在大丽轮枝菌（Verticillium dahliae）中发现了一种对植物细胞具有毒性的真菌效应子。该效应子包含几个真菌胞外膜蛋白（CFEM）结构域中的一个非典型共价区、一个由四个富含脯氨酸的 14 氨基酸重复序列组成的串联重复区以及一个 C 端区，因此被命名为大丽轮枝菌四肽重复蛋白（VdTRP）。VdTRP 的膜靶向性对其细胞毒性至关重要。CFEM 介导膜靶向，而串联重复区则在细胞膜上发挥毒性功能。几丁质酶样 1（CTL1）是棉花的一种重要细胞凋亡蛋白，可将 VdTRP 从细胞膜转向细胞凋亡体。在不影响棉花生长发育的情况下，过表达 CTL1 的转基因棉花大大增强了棉花对大丽花病毒的抗性，这意味着 CTL1 有可能应用于培育具有高抗枯萎病能力的棉花。我们的数据表明，对效应目标的遗传操作是提高作物对真菌病原体抗性的潜在策略。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.