EXPLORING PLANT VIRUS DIVERSITY IN WASTEWATER AND RECLAIMED WATER THROUGH METAGENOMIC ANALYSIS

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2024-11-22 DOI:10.1016/j.watres.2024.122827

Enric Cuevas-Ferrando, Gloria Sánchez, Alba Pérez-Cataluña

{"title":"EXPLORING PLANT VIRUS DIVERSITY IN WASTEWATER AND RECLAIMED WATER THROUGH METAGENOMIC ANALYSIS","authors":"Enric Cuevas-Ferrando, Gloria Sánchez, Alba Pérez-Cataluña","doi":"10.1016/j.watres.2024.122827","DOIUrl":null,"url":null,"abstract":"The use of reclaimed water for agricultural activities is being widely employed to address drought and water scarcity. Nevertheless, the disinfection processes do not consistently facilitate the complete removal of all eukaryotic viruses within these reclaimed waters. Consequently, it may pose a risk not only to humans but also to irrigated plants. We analyzed 48 influent and 48 effluent samples from 4 different wastewater treatment plants (WWTPs) by high-throughput sequencing (HTS) to characterize plant-associated virome over a one-year period. Our results showed high levels of plant viruses in both influent and effluent waters. The predominant family identified was <em>Virgaviridae</em>, recognized for its high environmental persistence. Notably, the identification of Tomato Brown Rugose Fruit virus (ToBRFV), classified as a harmful organism by the European Union and subject to strict containment measures to control its spread, highlights the importance of monitoring reclaimed water to mitigate the spread of such viruses into the environment. These findings underscore the need of analyzing reclaimed water from a One Health perspective, ensuring its safety for humans, animals, plants, and the environment alike.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"71 1","pages":""},"PeriodicalIF":11.4000,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.watres.2024.122827","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

The use of reclaimed water for agricultural activities is being widely employed to address drought and water scarcity. Nevertheless, the disinfection processes do not consistently facilitate the complete removal of all eukaryotic viruses within these reclaimed waters. Consequently, it may pose a risk not only to humans but also to irrigated plants. We analyzed 48 influent and 48 effluent samples from 4 different wastewater treatment plants (WWTPs) by high-throughput sequencing (HTS) to characterize plant-associated virome over a one-year period. Our results showed high levels of plant viruses in both influent and effluent waters. The predominant family identified was Virgaviridae, recognized for its high environmental persistence. Notably, the identification of Tomato Brown Rugose Fruit virus (ToBRFV), classified as a harmful organism by the European Union and subject to strict containment measures to control its spread, highlights the importance of monitoring reclaimed water to mitigate the spread of such viruses into the environment. These findings underscore the need of analyzing reclaimed water from a One Health perspective, ensuring its safety for humans, animals, plants, and the environment alike.

查看原文本刊更多论文

通过元基因组分析探索废水和再生水中的植物病毒多样性

为解决干旱和缺水问题，农业活动正在广泛使用再生水。然而，消毒过程并不能完全清除再生水中的所有真核病毒。因此，这不仅会对人类造成危害，也会对灌溉植物造成危害。我们通过高通量测序（HTS）分析了来自 4 个不同污水处理厂（WWTPs）的 48 份进水和 48 份出水样本，以确定一年内植物相关病毒组的特征。结果表明，进水和出水中都含有大量植物病毒。鉴定出的主要病毒科为病毒科（Virgaviridae），因其在环境中的高持久性而得到公认。值得注意的是，我们发现了番茄褐芦果病毒（ToBRFV），该病毒被欧盟列为有害生物，必须采取严格的遏制措施来控制其传播，这凸显了监测再生水以减少此类病毒在环境中传播的重要性。这些发现强调了从 "一体健康 "的角度分析再生水的必要性，以确保其对人类、动物、植物和环境的安全性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.