Overlooked roles of improved substrates functions in remodeling microbial community and driving metabolic traits during sludge fermentation triggered by surfactants and antibiotics co-existence

Abstract

Sludge anaerobic fermentation is a pivotal route to transit wastewater treatment plants towards energy-neutral and resource recovery-oriented plants, while the overall efficiency is commonly restrained by co-exist contaminants. This study unveiled the cooperative effect of sulfadiazine (SDZ) and linear alkylbenzene sulfonates (LAS) in driving volatile fatty acids (VFAs) biosynthesis during waste activated sludge (WAS) anaerobic fermentation. The VFAs remarkably elevated to 1189 mg COD/L in LAS/SDZ, which was approximately 1.3–3.2 folds of SDZ and LAS. SDZ and LAS synergistically disintegrated extracellular polymeric substances to provide fermentation substrates. These changes altered microbial population and enhanced microbial networks interconnection. Moreover, the proteins released from WAS might facilitate electron transfer among microorganisms. The functional anaerobic species, including hydrolytic-acidogenic bacteria (e.g., Veillonellaceae and Lactobacillus) and electroactive bacteria (e.g., Parabacteroides and Fonticella), were enriched. Also, the metabolic traits responsible for transmembrane transport, intracellular metabolism, VFAs biosynthesis and electron transfer-related process were strengthened with the upregulation of functional genes. Further analysis revealed that the functional anaerobic species activated quorum sensing and two-component systems to counteract unfavorable LAS/SDZ stress and maintain high metabolic activities. This work elucidated the overlooked roles of substrates in modulating anaerobic consortia and metabolic traits in sludge fermentation systems with mixed pollutants stressing.