Haoran Yu, Ziqing Qiang, Yelin Sun, Mengyao Sun, Lei Zhang, Bohao Yu, Wen Lei and Weibing Zhang
{"title":"A novel ratiometric fluorescent probe based on an internal reference of lanthanide/nucleotide for alkaline phosphatase detection†","authors":"Haoran Yu, Ziqing Qiang, Yelin Sun, Mengyao Sun, Lei Zhang, Bohao Yu, Wen Lei and Weibing Zhang","doi":"10.1039/D4AN01341G","DOIUrl":null,"url":null,"abstract":"<p >Based on the specific hydrolytic ability of alkaline phosphatase (ALP), a novel biocompatible ratiometric lanthanide fluorescent probe based on an internal reference (CIP@SiO<small><sub>2</sub></small>-Ce/ATP-Tris) was constructed with Ce<small><sup>3+</sup></small> as the central ion, adenosine triphosphate (ATP) as the ligand, Tris as the auxiliary ligand and ciprofloxacin (CIP) encapsulated into SiO<small><sub>2</sub></small> nanoparticles as the reference signal. The fluorescent probe emits characteristic fluorescence at 363 nm belonging to Ce<small><sup>3+</sup></small> as the working signal and at 435 nm belonging to CIP as the reference signal. Dephosphorylation disrupted the coordination of Ce/ATP-Tris with the enzymatic reaction of ALP, which resulted in fluorescence quenching of Ce<small><sup>3+</sup></small>. The reference fluorescence was kept stable because of the protective effect of encapsulation by SiO<small><sub>2</sub></small>. The biosensor analysis method was achieved by comparing the relationship between <em>I</em><small><sub>435</sub></small>/<em>I</em><small><sub>363</sub></small> and ALP concentration. The detection limit is 0.0025 U L<small><sup>−1</sup></small>, and the linear range of detection is 0.1–20 U L<small><sup>−1</sup></small>. It was subsequently used to detect ALP in samples of fetal bovine serum and human serum, and promising results were obtained.</p>","PeriodicalId":63,"journal":{"name":"Analyst","volume":" 1","pages":" 87-93"},"PeriodicalIF":3.6000,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analyst","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/an/d4an01341g","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Based on the specific hydrolytic ability of alkaline phosphatase (ALP), a novel biocompatible ratiometric lanthanide fluorescent probe based on an internal reference (CIP@SiO2-Ce/ATP-Tris) was constructed with Ce3+ as the central ion, adenosine triphosphate (ATP) as the ligand, Tris as the auxiliary ligand and ciprofloxacin (CIP) encapsulated into SiO2 nanoparticles as the reference signal. The fluorescent probe emits characteristic fluorescence at 363 nm belonging to Ce3+ as the working signal and at 435 nm belonging to CIP as the reference signal. Dephosphorylation disrupted the coordination of Ce/ATP-Tris with the enzymatic reaction of ALP, which resulted in fluorescence quenching of Ce3+. The reference fluorescence was kept stable because of the protective effect of encapsulation by SiO2. The biosensor analysis method was achieved by comparing the relationship between I435/I363 and ALP concentration. The detection limit is 0.0025 U L−1, and the linear range of detection is 0.1–20 U L−1. It was subsequently used to detect ALP in samples of fetal bovine serum and human serum, and promising results were obtained.