Enhanced therapeutic efficacy of granulocyte/monocyte adsorption in rats with drug-induced colitis : Insights from a downsized bead column and newly formed B cells.
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引用次数: 0
Abstract
Introduction: Granulocyte/monocyte adsorption therapy can manage mild-to-moderate inflammatory bowel disease by removing activated granulocytes and monocytes. We evaluated granulocyte/monocyte adsorption using new columns with reduced bead size and theoretically enhanced adsorption.
Methods: We assessed granulocyte/monocyte adsorption in rats with colitis by analyzing cell changes and cytokine production.
Results: Granulocyte/monocyte adsorption with the new columns improved histology in rats with colitis. Contrary to expectations, the adsorption rate of granulocytes/monocytes into the blood did not show a significant improvement. However, flow cytometry showed increased B cells in peripheral blood mononuclear cells and newly formed B cells in the bone marrow, which produced more interleukin-10 than peripheral blood B cells. Newly formed B cells adoptively transferred into colitis rats accumulated at the inflammation site and tended to inhibit intestinal shortening.
Conclusions: Newly formed B cells with strong interleukin-10 production may alleviate inflammation. The new columns suggest potential for controlling colitis.
引言粒细胞/单核细胞吸附疗法可清除活化的粒细胞和单核细胞，从而控制轻度至中度炎症性肠病。我们评估了使用新型色谱柱对粒细胞/单核细胞的吸附情况，这种色谱柱的珠子尺寸更小，理论上吸附能力更强：我们通过分析细胞变化和细胞因子的产生来评估结肠炎大鼠的粒细胞/单核细胞吸附情况：结果：新型色谱柱对粒细胞/单核细胞的吸附改善了结肠炎大鼠的组织学状况。与预期相反，血液中粒细胞/单核细胞的吸附率并没有明显改善。不过，流式细胞术显示，外周血单核细胞中的 B 细胞和骨髓中新形成的 B 细胞有所增加，后者比外周血 B 细胞产生更多的白细胞介素-10。被收养移植到结肠炎大鼠体内的新形成的B细胞在炎症部位聚集，并有抑制肠道缩短的趋势：结论：新形成的 B 细胞能产生大量白细胞介素-10，可缓解炎症。结论：白细胞介素-10分泌旺盛的新形成的B细胞可缓解炎症。
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