A Rhodamine-based high-sensitivity low-cytotoxicity probe for rapid turn-on detection of Hg2+

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-11-16 DOI:10.1016/j.saa.2024.125443

Yongdi Liang , Xiaofeng Wang , Meixi Chen , Brian Musikavanhu , Attiq Ur Rehman , Xiuli Wang , Zhaoli Xue , Long Zhao

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Abstract

By integrating Rhodamine B and 4-phenylmorpholine moieties, a novel fluorescent probe named RhPy is synthesized for detecting Hg²⁺. Its recognition mechanism involves the reaction of Hg²⁺ with dithiooxamide, ultimately triggering the opening of the Rhodamine spirolactam and forming a new molecule RhPy-S with strong emission. The probe exhibits impressive limit of detection (0.015 μM) and short response time (<10 s). Importantly, RhPy shows almost none-cytotoxicity and RhPy-S has the emission spectrum peaking at 596 nm, which endow the probe with a good tissue penetration ability and practical utility in living cells, zebrafish and in vivo mice models. This work advances the field by providing a highly sensitive chemosensor for both environmental and biological applications.

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一种基于罗丹明的高灵敏度、低毒性探针，用于快速开启 Hg2 检测。

通过整合罗丹明 B 和 4-苯基吗啉分子，合成了一种用于检测 Hg2+ 的新型荧光探针 RhPy。它的识别机制包括 Hg2+ 与二硫代草酰氨反应，最终引发罗丹明螺内酰胺打开，形成具有强发射的新分子 RhPy-S。该探针具有极高的检测限（0.015 μM）和较短的响应时间（0.015 μM）。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.