The Effect of NiTi Brush, Polishing Brush, and Chemical Agent on the Dental Implant Surface Morphology and Cytocompatibility

IF 3.7 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Implant Dentistry and Related Research Pub Date : 2024-11-21 DOI:10.1111/cid.13417

Giulia Brunello, Kathrin Becker, Nicole Rauch, Frank Schwarz, Jürgen Becker

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引用次数: 0

Abstract

Objectives

To in vitro investigate the effect of different implant surface decontamination methods and treatment storing conditions on implant surface morphology and cell viability.

Materials and Methods

Titanium disks with a sand-blasted and acid-etched surface (Promote, PRO) were treated with diamond polishing brushes (BRUSH), nickel-titanium brushes (NITI), or phenol and sulfuric acid-gel (GEL). The disks were stored in saline (-S) or left exposed to air overnight (-A). Untreated (PRO) and machined (MACHINED) disks were used as controls. GEL samples were treated for the 60 s, while the operative time was recorded for BRUSH and NITI. The samples were subjected to scanning electron microscopy (SEM), surface roughness measurements, and cell viability (SaOS-2 cells, 7 days) assessment.

Results

The operative time was shorter for NITI than for BRUSH (p = 0.017). The original surface morphology (PRO) was not altered in the GEL group, in contrast with what was observed for BRUSH and NITI. The type of storage did not influence the surface morphology. No significant differences in Sa and Sz were observed among the groups, except for MACHINED, which presented lower Sa values (p < 0.05). Cells were able to proliferate on all surfaces. NITI-S showed significantly higher cell viability compared to all groups (p ≤ 0.001), except for NITI-A and MACHINED. Among the treated groups, only one additional significant difference was found, as NITI-A performed better than GEL-S.

Conclusions

None of the investigated protocols compromised the cytocompatibility of the titanium dental implant surface. The best results were registered in the NITI group when the samples were stored in saline. Future studies should confirm the effectiveness of the proposed methods in removing bacterial biofilm from contaminated implant surfaces.

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镍钛刷、抛光刷和化学剂对牙科种植体表面形态和细胞相容性的影响

目的：体外研究不同种植体表面净化方法和处理储存条件对种植体表面形态和细胞活力的影响：体外研究不同种植体表面去污方法和处理储存条件对种植体表面形态和细胞活力的影响：用金刚石抛光刷（BRUSH）、镍钛刷（NITI）或苯酚和硫酸凝胶（GEL）对表面喷砂和酸蚀的钛盘（Promote，PRO）进行处理。磁盘储存在生理盐水中（-S）或暴露在空气中过夜（-A）。未经处理（PRO）和机加工（MACHINED）的磁盘用作对照组。GEL 样品的处理时间为 60 秒，而 BRUSH 和 NITI 则记录了操作时间。对样品进行扫描电子显微镜（SEM）、表面粗糙度测量和细胞存活率（SaOS-2 细胞，7 天）评估：结果：NITI 的手术时间比 BRUSH 短（p = 0.017）。GEL 组的原始表面形态（PRO）没有改变，这与 BRUSH 和 NITI 观察到的情况相反。储存类型对表面形态没有影响。除 MACHINED 组的 Sa 值较低外，其他各组的 Sa 和 Sz 均无明显差异（p 结论）：所有研究方案都不会影响钛种植体表面的细胞相容性。将样本保存在生理盐水中时，NITI 组的结果最好。未来的研究应确认所建议的方法在清除受污染种植体表面的细菌生物膜方面的有效性。

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来源期刊

Clinical Implant Dentistry and Related Research 医学-牙科与口腔外科

CiteScore

6.00

自引率

13.90%

发文量

103

审稿时长

4-8 weeks

期刊介绍： The goal of Clinical Implant Dentistry and Related Research is to advance the scientific and technical aspects relating to dental implants and related scientific subjects. Dissemination of new and evolving information related to dental implants and the related science is the primary goal of our journal. The range of topics covered by the journals will include but be not limited to: New scientific developments relating to bone Implant surfaces and their relationship to the surrounding tissues Computer aided implant designs Computer aided prosthetic designs Immediate implant loading Immediate implant placement Materials relating to bone induction and conduction New surgical methods relating to implant placement New materials and methods relating to implant restorations Methods for determining implant stability A primary focus of the journal is publication of evidenced based articles evaluating to new dental implants, techniques and multicenter studies evaluating these treatments. In addition basic science research relating to wound healing and osseointegration will be an important focus for the journal.