A turn-on AIE dual-channel fluorescent probe for sensing Cr³⁺/ClO^- and application in cell imaging.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2024-11-17 DOI:10.1016/j.saa.2024.125444

Huizhen Wang, Yan Tang, Kezhen Gou, Dingxin Qiu, Cheng Du, Zhengfeng Xie, Guanjun Chang

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Abstract

A Cr³⁺/ClO^--enhanced fluorescent probe, DNS (5-(dimethylamino)-N'-(2-hydroxy-4,6-dimethoxybenzylidene)-naphthalene-1-sulfonyl hydrazide), with aggregation-induced emission (AIE) properties was synthesized using dansylhydrazide and 4,6-dimethoxysalicylaldehyde as starting materials. The probe rapidly and selectively detects Cr³⁺ and ClO^- in a solvent system of H₂O/DMSO (2:8). Upon binding with Cr³⁺/ClO^-, the probe exhibits a significant fluorescence enhancement, with minimal interference from other ions. The detection limits (LOD) were determined to be 5.36 × 10^-7 mol/L for Cr³⁺ and 3.65 × 10^-7 mol/L for ClO^-. The binding mechanisms of DNS with Cr³⁺/ClO^- were investigated through Job's plot, 1H NMR titration, and mass spectrometry. Furthermore, the probe's low cytotoxicity and biocompatibility suggest its potential for detecting exogenous Cr³⁺/ClO^- and endogenous ClO^- in living cells. DNS shows promise for real-time detection and bioimaging applications.

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用于感测 Cr3+/ClO- 的开启式 AIE 双通道荧光探针及其在细胞成像中的应用。

以丹酰肼和 4,6-二甲氧基水杨醛为起始原料，合成了一种具有聚集诱导发射（AIE）特性的 Cr3+/ClO- 增强荧光探针 DNS（5-(dimethylamino)-N'-(2-hydroxy-4,6-dimethoxybenzylidene)-naphthalene-1-sulfonyl hydrazide）。该探针可在 H2O/DMSO (2:8) 溶剂体系中快速、选择性地检测 Cr3+ 和 ClO-。与 Cr3+/ClO- 结合后，探针的荧光明显增强，其他离子的干扰极小。经测定，Cr3+ 的检测限（LOD）为 5.36 × 10-7 mol/L，ClO- 的检测限（LOD）为 3.65 × 10-7 mol/L。通过约伯图、1H NMR 滴定和质谱分析，研究了 DNS 与 Cr3+/ClO- 的结合机制。此外，该探针的低细胞毒性和生物相容性表明，它具有在活细胞中检测外源 Cr3+/ClO- 和内源 ClO- 的潜力。DNS 显示了实时检测和生物成像应用的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

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