Total carbohydrate consumption through co-fermentation of agro-industrial waste: use of wild-type bacterial isolates specialized in the conversion of C-5 sugars to high levels of lactic acid with concomitant metabolization of toxic compounds.

IF 4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2024-11-21 DOI:10.1007/s11274-024-04202-9

Sheila Montipó, Elisa Bellan Menegussi, Marli Camassola, Ola Wallberg, Mats Galbe

{"title":"Total carbohydrate consumption through co-fermentation of agro-industrial waste: use of wild-type bacterial isolates specialized in the conversion of C-5 sugars to high levels of lactic acid with concomitant metabolization of toxic compounds.","authors":"Sheila Montipó, Elisa Bellan Menegussi, Marli Camassola, Ola Wallberg, Mats Galbe","doi":"10.1007/s11274-024-04202-9","DOIUrl":null,"url":null,"abstract":"Value-added bioproducts are linked to the expansion of lignocellulosic biorefineries based on agro-industrial waste and local economic growth. Thus, the aim of this study was to pretreat rice hull (RH), a highly recalcitrant biomass, with saturated steam and convert it to lactic acid (LA). Strategically, the individual fractions and the blend of detoxified liquor and water-insoluble solids were used as substrate in the simultaneous saccharification and co-fermentation (SSCF) by wild-type bacteria. The microbial consortium between Pediococcus acidilactici and Acetobacter cerevisiae enabled the metabolization of all the xylose contained in the liquor, as well as the consumption of all minor sugars when using the blend. Assays resulted in the production of 106.2 g L- 1 of LA. Furthermore, A. cerevisiae promoted complete degradation of 5-HMF/furfural in a short period of time. This study demonstrates the benefits provided by processes integration (SSCF/blend) employing high solids load (22% w/v), representing an innovative and economically interesting approach.","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":"40 12","pages":"388"},"PeriodicalIF":4.0000,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"World journal of microbiology & biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s11274-024-04202-9","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Value-added bioproducts are linked to the expansion of lignocellulosic biorefineries based on agro-industrial waste and local economic growth. Thus, the aim of this study was to pretreat rice hull (RH), a highly recalcitrant biomass, with saturated steam and convert it to lactic acid (LA). Strategically, the individual fractions and the blend of detoxified liquor and water-insoluble solids were used as substrate in the simultaneous saccharification and co-fermentation (SSCF) by wild-type bacteria. The microbial consortium between Pediococcus acidilactici and Acetobacter cerevisiae enabled the metabolization of all the xylose contained in the liquor, as well as the consumption of all minor sugars when using the blend. Assays resulted in the production of 106.2 g L^- 1 of LA. Furthermore, A. cerevisiae promoted complete degradation of 5-HMF/furfural in a short period of time. This study demonstrates the benefits provided by processes integration (SSCF/blend) employing high solids load (22% w/v), representing an innovative and economically interesting approach.

查看原文本刊更多论文

通过共同发酵农用工业废物消耗碳水化合物总量：使用专门将 C-5 糖转化为高浓度乳酸并同时代谢有毒化合物的野生型细菌分离物。

高附加值的生物产品与以农用工业废料为基础的木质纤维素生物炼制厂的扩张和地方经济增长息息相关。因此，本研究的目的是用饱和蒸汽预处理稻壳（RH）这种高度难降解的生物质，并将其转化为乳酸（LA）。从战略上讲，在野生型细菌的同步糖化和共发酵（SSCF）过程中，单个馏分和解毒液与水不溶性固体的混合物被用作底物。酸乳球菌（Pediococcus acidilactici）和醋酸纤维菌（Acetobacter cerevisiae）组成的微生物联合体能够代谢白酒中的所有木糖，并在使用混合液时消耗所有次要糖类。实验结果表明，LA 的产量为 106.2 g L-1。此外，A. cerevisiae 还能在短时间内促进 5-HMF/糠醛的完全降解。这项研究证明了采用高固体负荷（22% w/v）的工艺整合（SSCF/混合）所带来的益处，是一种具有创新性和经济意义的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.