Abnormal Expression of lncRNA SNHG7 in Dry Eye Disease and Its Effect on Human Conjunctival Epithelial Cells.

IF 1.6 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Tohoku Journal of Experimental Medicine Pub Date : 2025-06-20 Epub Date: 2024-11-21 DOI:10.1620/tjem.2024.J134
Shuang Liu, Guang Yang, Qingnan Li, Shaohua Tang
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引用次数: 0

Abstract

Analyzing the pathogenic mechanism of dry eye disease provides a new research point for the treatment of patients. A total of 86 patients with dry eye disease and the same number of healthy individuals were recruited as the patient group and normal group. The levels of small nucleolar RNA host gene 7 (SNHG7) in tear samples and human conjunctival epithelial cells (HCECs) were determined by a quantitative real-time PCR (RT-qPCR). Meanwhile, TNF-α and IL-6 mRNA levels in HCECs were also detected by RT-qPCR. The molecular mechanism of SNHG7 was analyzed by biological prediction and luciferase activity assay. The correlation between the expression of SNHG7 and microRNA-146a-5p (miR-146a-5p) with clinical indicators in dry eye disease patients using Pearson analysis. SNHG7 expression in tears samples and HCECs from dry eye disease patients was significantly increased (P < 0.001). The levels of TNF-α mRNA and IL-6 mRNA were upregulated in the HCECs model group while silencing SNHG7 suppressed their expression (P < 0.05). SNHG7 level was negatively correlated with miR-146a-5p, break-up time (BUT) and Schirmer's test (SIT) value, while positively correlated with corneal fluorescein staining (CFS) score (P < 0.001). miR-146a-5p was positively proportional to BUT and SIT, and inversely proportional to CFS score (P < 0.001). SNHG7 expression was enhanced, while miR-146a-5p expression was decreased in dry eye disease. Down-regulation of SNHG7 reduced the level of inflammation in HCECs. SNHG7 and miR-146a-5p were closely related to the development of disease, probably as biological targets for treatment in dry eye disease.

干眼症中 lncRNA SNHG7 的异常表达及其对人类结膜上皮细胞的影响
分析干眼病的发病机制,为干眼病患者的治疗提供了新的研究点。共招募了86名干眼病患者和相同数量的健康个体作为患者组和正常组。采用实时荧光定量PCR (RT-qPCR)检测泪液样品和人结膜上皮细胞(HCECs)中小核仁RNA宿主基因7 (SNHG7)的表达水平。同时采用RT-qPCR检测HCECs中TNF-α、IL-6 mRNA水平。通过生物学预测和荧光素酶活性测定分析SNHG7的分子机制。采用Pearson分析干眼病患者SNHG7和microRNA-146a-5p (miR-146a-5p)表达与临床指标的相关性。SNHG7在干眼病患者泪液和HCECs中的表达显著升高(P < 0.001)。HCECs模型组TNF-α mRNA和IL-6 mRNA表达上调,SNHG7沉默抑制其表达(P < 0.05)。SNHG7水平与miR-146a-5p、破裂时间(BUT)、Schirmer’s test (SIT)值呈负相关,与角膜荧光素染色(CFS)评分呈正相关(P < 0.001)。miR-146a-5p与BUT、SIT呈正相关,与CFS评分呈负相关(P < 0.001)。干眼症患者SNHG7表达增强,miR-146a-5p表达降低。SNHG7的下调降低了HCECs的炎症水平。SNHG7和miR-146a-5p与疾病的发展密切相关,可能是干眼病治疗的生物学靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.60
自引率
4.50%
发文量
171
审稿时长
1 months
期刊介绍: Our mission is to publish peer-reviewed papers in all branches of medical sciences including basic medicine, social medicine, clinical medicine, nursing sciences and disaster-prevention science, and to present new information of exceptional novelty, importance and interest to a broad readership of the TJEM. The TJEM is open to original articles in all branches of medical sciences from authors throughout the world. The TJEM also covers the fields of disaster-prevention science, including earthquake archeology. Case reports, which advance significantly our knowledge on medical sciences or practice, are also accepted. Review articles, Letters to the Editor, Commentary, and News and Views will also be considered. In particular, the TJEM welcomes full papers requiring prompt publication.
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