Healing effect of curcumin on tooth extraction sockets in diabetic rats.

IF 2.2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Applied Oral Science Pub Date : 2024-11-15 eCollection Date: 2024-01-01 DOI:10.1590/1678-7757-2024-0251

Tipthanan Chotipinit, Weera Supronsinchai, Soranun Chantarangsu, Supaporn Suttamanatwong

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引用次数: 0

Abstract

Objective: Diabetes mellitus (DM) delays wound healing, including those following tooth extractions. Curcumin (CCM) can promote soft tissue and bone healing. The present study investigates the healing effects of CCM on tooth extraction sockets in diabetic rats.

Methodology: Ninety-six male Wistar rats were divided into the following four groups: Control+Corn Oil (CO), Control+CCM, DM+CO, and DM+CCM. Each group was subdivided into 7-, 14-, and 28-day time point subgroups comprising eight rats. All animals had their maxillary first molars extracted. CCM-treated rats received 100 mg/kg of CCM orally for 7, 14, and 28 days. The lesion area was evaluated using macroscopic analyses, whereas socket healing was assessed by hematoxylin and eosin staining. Keratinocyte growth factor (KGF), Runt-related transcription factor 2 (Runx2), and collagen type I (COL1) expression levels were obtained using quantitative polymerase chain reaction (qPCR). Bone healing was analyzed by means of microcomputed tomography (μCT).

Results: After 7 days, the groups showed no significant differences in lesion area and by day 14, no lesions were present. CCM treatment increased KGF mRNA expression in diabetic rats; however, diabetic rats showed delayed bone healing unrelated to CCM. CCM treatment resulted in increased Runx2 mRNA expression only in control rats, whereas COL1 mRNA expression remained unaffected by CCM.

Conclusion: CCM shows potential as a soft tissue healing enhancer in diabetic rats and could serve as an additional treatment to promote soft tissue repair in diabetic individuals. Although CCM did not impact alveolar bone healing, it may enhance bone healing in other skeleton regions.

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姜黄素对糖尿病大鼠拔牙窝的愈合作用

目的：糖尿病（DM）会延迟伤口愈合，包括拔牙后的伤口愈合。姜黄素（CCM）可促进软组织和骨愈合。本研究探讨了姜黄素对糖尿病大鼠拔牙窝的愈合效果：将 96 只雄性 Wistar 大鼠分为以下四组：对照组+玉米油（CO）、对照组+中药、DM+CO 和 DM+中药。每组又分为 7 天、14 天和 28 天时间点分组，每组八只大鼠。所有动物的上颌第一臼齿均已拔除。大鼠在 7、14 和 28 天内口服 100 毫克/千克的 CCM。病变面积通过宏观分析进行评估，而窝沟愈合则通过苏木精和伊红染色进行评估。角质细胞生长因子（KGF）、Runt 相关转录因子 2（Runx2）和 I 型胶原（COL1）的表达水平通过定量聚合酶链反应（qPCR）获得。通过微计算机断层扫描（μCT）分析骨愈合情况：结果：7 天后，各组的病变面积无明显差异，第 14 天时，各组均无病变。CCM 治疗增加了糖尿病大鼠的 KGF mRNA 表达，但糖尿病大鼠的骨愈合延迟与 CCM 无关。CCM 治疗仅导致对照组大鼠 Runx2 mRNA 表达增加，而 COL1 mRNA 表达不受 CCM 影响：结论：CCM 具有促进糖尿病大鼠软组织愈合的潜力，可作为促进糖尿病患者软组织修复的额外治疗方法。虽然 CCM 不会影响牙槽骨愈合，但它可能会促进其他骨骼区域的骨愈合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.