Minibioreactor arrays to model microbiome response to alcohol and tryptophan in the context of alcohol-associated liver disease.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2024-11-20 DOI:10.1038/s41522-024-00602-9

Wanchao Hu, Sabrine Naimi, Nicolas Trainel, Vanessa Liévin-Le Moal, Gabriel Perlemuter, Benoit Chassaing, Dragos Ciocan, Anne-Marie Cassard

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Abstract

The intestinal microbiota (IM) plays a role in the severity of alcohol-associated liver disease. Modifying severe alcohol-associated hepatitis (AH) dysbiosis improves liver injury through tryptophan (Trp) metabolites and the aryl hydrocarbon receptor (AhR). However, Trp's effect on the IM in alcohol use disorder (AUD) patients remains unclear. Here, we used an in vitro microbiota modeling system named Minibioreactor arrays (MBRAs). Feces from AUD patients with or without AH were treated with low, normal, or high Trp concentrations, with subsequent treatment with alcohol. Microbiota composition and AhR activity were studied. We showed that microbial communities from donors were maintained in MBRAs. High and low Trp increased the abundance of pathogen Escherichia Shigella. In the absence of alcohol, Trp changed more bacteria in AUD IM compared to AH IM. Normal Trp increased the AhR activity. Overall, maintaining normal Trp levels may prevent dysbiosis in AUD or AH, pending in vivo confirmation.

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用微型生物反应器阵列模拟酒精相关肝病中微生物组对酒精和色氨酸的反应。

肠道微生物群（IM）在酒精相关性肝病的严重程度中发挥着作用。通过色氨酸（Trp）代谢物和芳基烃受体（AhR）改变严重的酒精相关性肝炎（AH）菌群失调，可改善肝损伤。然而，Trp对酒精使用障碍（AUD）患者的IM的影响仍不清楚。在这里，我们使用了一种名为微型生物反应器阵列（MBRAs）的体外微生物群建模系统。用低浓度、正常浓度或高浓度的 Trp 处理有或无 AH 的 AUD 患者的粪便，然后再用酒精处理。对微生物群组成和 AhR 活性进行了研究。我们发现，来自供体的微生物群落在 MBRA 中得以维持。高浓度和低浓度 Trp 会增加病原体志贺氏菌的数量。在没有酒精的情况下，与 AH IM 相比，Trp 改变了 AUD IM 中更多的细菌。正常的 Trp 会增加 AhR 的活性。总之，保持正常的 Trp 水平可防止 AUD 或 AH 中的菌群失调，但这有待体内证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.