DNA Aptamers That Bind to Alginate Hydrogels.

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-11-21 DOI:10.1021/acsbiomaterials.4c01436

Ali Parvez, Dana A Baum

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引用次数: 0

Abstract

Hydrogels have become common in wound treatment because they form very stable and biocompatible environments that promote healing. However, due to the highly porous hydrogel structure, any therapeutic added to these gels tends to diffuse quickly and impact delivery to the target site. Aptamers are short, single-stranded DNA or RNA sequences that bind specifically to a target, so aptamers that bind to hydrogels could serve as tags for therapeutics to prevent rapid diffusion and allow for extended delivery. An in vitro selection approach was developed to identify DNA aptamers for alginate hydrogels. Two DNA aptamers were shown to bind hydrogels ranging from 0.5 to 2% alginate and could be either encapsulated during gelation or introduced to preformed gels. Both aptamers also showed specificity for binding to alginate compared to agarose. To demonstrate the functional aspect of the aptamers as tethers for other biomolecules, both aptamers were conjugated to BSA. Aptamer-conjugated BSA was retained longer in the hydrogel during week-long diffusion studies both when encapsulated or introduced to preformed gels, which adds flexibility to how these aptamers can be deployed in a clinical setting.

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与藻酸盐水凝胶结合的 DNA 短聚物。

水凝胶能形成非常稳定和生物兼容的环境，促进伤口愈合，因此已成为伤口治疗的常用材料。然而，由于水凝胶的结构具有高度多孔性，添加到这些凝胶中的任何治疗剂往往会迅速扩散，影响向目标部位的输送。适配体是短的单链 DNA 或 RNA 序列，能与靶点特异性结合，因此与水凝胶结合的适配体可作为治疗药物的标签，防止快速扩散并延长给药时间。研究人员开发了一种体外筛选方法，以鉴定适用于海藻酸盐水凝胶的DNA适配体。结果表明，两种DNA适配体能与0.5%至2%的藻酸盐水凝胶结合，既可在凝胶化过程中封装，也可引入预成型凝胶。与琼脂糖相比，这两种适配体还显示出与藻酸盐结合的特异性。为了证明适配体作为其他生物大分子拴系物的功能，两种适配体都与 BSA 连接。在为期一周的扩散研究中，无论是封装还是引入预成型凝胶，肽聚体轭合的 BSA 在水凝胶中的保留时间都更长，这为这些肽聚体在临床环境中的应用增加了灵活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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