Foliar application of myo-inositol inhibits the growth of trifoliate orange (Poncirus trifoliata) seedlings by inducing alterations in cell wall structure and energy metabolism disorders
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引用次数: 0
Key message
Exogenous myo-inositol induces alterations in leaf cell wall structure and energy metabolism disorders, which may contribute to growth inhibition of trifoliate orange seedlings.
Abstract
Myo-inositol (MI) is widely distributed in all living organisms as a central molecule. In plants, de novo biosynthesis of MI is known to function in multiple aspects of plant growth and development. However, the consequences of MI accumulation in regulating plant growth, especially by exogenous supply, have received little attention. In this study, we characterized cell wall structure, target metabolites in energy metabolism, and gene expression changes related to MI metabolism after foliar application of MI to trifoliate orange seedlings. We observed that exogenous MI increases leaf and root MI levels and inhibits plant growth and dry mass accumulation in trifoliate orange seedlings. Additionally, we detected the downregulation of PtrMIPS and upregulation of PtrMIOXs in leaves due to the application of MI. Structural characterization and targeted metabolomics analysis revealed that exogenous MI induces alterations in leaf cell wall structure and energy metabolism disorders, which may contribute to growth inhibition of trifoliate orange seedlings. We propose and discuss a model to explain how exogenous MI exerts a negative impact on trifoliate orange seedling growth.
关键信息外源肌醇诱导叶片细胞壁结构改变和能量代谢紊乱，这可能是三叶橙幼苗生长抑制的原因之一。 摘要肌醇（MI）作为一种核心分子广泛分布于所有生物体内。在植物中，已知 MI 的从头生物合成在植物生长和发育的多个方面发挥作用。然而，MI 的积累对植物生长的调节作用，尤其是通过外源供应调节植物生长的作用，却鲜有人关注。在本研究中，我们对三叶橙幼苗叶面施用 MI 后的细胞壁结构、能量代谢中的目标代谢物以及与 MI 代谢相关的基因表达变化进行了表征。我们观察到，外源 MI 会增加三叶橙幼苗叶片和根部的 MI 含量，抑制植株生长和干物质积累。此外，我们还发现施用 MI 后，叶片中的 PtrMIPS 下调，PtrMIOX 上调。结构表征和靶向代谢组学分析表明，外源 MI 会诱导叶片细胞壁结构的改变和能量代谢紊乱，这可能是导致三叶橙幼苗生长受抑制的原因之一。我们提出并讨论了一个模型来解释外源 MI 如何对三叶橙幼苗的生长产生负面影响。
期刊介绍：
Trees - Structure and Function publishes original articles on the physiology, biochemistry, functional anatomy, structure and ecology of trees and other woody plants. Also presented are articles concerned with pathology and technological problems, when they contribute to the basic understanding of structure and function of trees. In addition to original articles and short communications, the journal publishes reviews on selected topics concerning the structure and function of trees.
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