CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles.

IF 3.8 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2024-11-19 DOI:10.1038/s41598-024-79866-2

Qianquan Chen, Yongqin Li, Zheng Fang, Qingshan Wu, Leitao Tan, Qingbei Weng

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引用次数: 0

Abstract

The bean beetle (Callosobruchus maculatus) clearly exhibits population density-dependent polymorphism. Cytochrome P450 (CYP) is involved in many aspects of the physiological activities of insects. However, the role of CYP in population density-dependent polymorphisms remains unknown. The terminal oocyte maturity rate of high-population-density individuals (H) was faster than that of low-population-density individuals (L). A total of 56 CYP-like genes were identified from transcriptomic and genomic data, including seven clan 2 CYP-like genes, seven mitochondrial CYP-like genes, 19 clan 3 CYP-like genes, and 23 clan 4 CYP-like genes. Gene duplication might occur in CYP9Z4-like, CYP345A1-like, CYP345A2-like, CYP349A1-like, CYP349A2-like, and CYP4BN4-like. Thirteen and two CYP-like genes were up-regulated and down-regulated, respectively, in H. Among these CYP-like genes, CYP4BN4v7-like was the most abundant CYP. CYP4BN4v7-like was more highly expressed in the head than in the thorax and abdomen. Its mRNA levels in the head, thorax, and abdomen were greater in H than in L. After RNA interference decreased its mRNA level, the terminal oocyte maturity rate decreased. Moreover, the expression level of insulin-like peptide 1 (ILP1), which plays a vital role in regulating terminal oocyte development, decreased in the head. In conclusion, CYP4BN4v7-like modulated the population density-dependent terminal oocyte maturity rate by regulating the expression of ILP1.

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CYP4BN4v7 可调节豆瓣菜中与种群密度相关的卵母细胞成熟率。

豆甲虫（Callosobruchus maculatus）明显表现出种群密度依赖性多态性。细胞色素 P450（CYP）参与了昆虫生理活动的许多方面。然而，CYP 在种群密度依赖性多态性中的作用仍然未知。高种群密度个体（H）的卵母细胞末期成熟速度快于低种群密度个体（L）。从转录组和基因组数据中共鉴定出 56 个类 CYP 基因，包括 7 个族 2 类 CYP 基因、7 个线粒体类 CYP 基因、19 个族 3 类 CYP 基因和 23 个族 4 类 CYP 基因。CYP9Z4-like 基因、CYP345A1-like 基因、CYP345A2-like 基因、CYP349A1-like 基因、CYP349A2-like 基因和 CYP4BN4-like 基因可能出现基因重复。在这些类 CYP 基因中，CYP4BN4v7-like 是含量最高的 CYP。与胸部和腹部相比，CYP4BN4v7-like 在头部的表达量更高。RNA 干扰降低其 mRNA 水平后，卵母细胞的末期成熟率下降。此外，在调节顶端卵母细胞发育中起重要作用的类胰岛素肽 1（ILP1）在头部的表达水平也有所下降。总之，CYP4BN4v7-like 通过调节 ILP1 的表达，调节了与种群密度相关的顶端卵母细胞成熟率。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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