{"title":"An Observational Case-control Study for BDNF Val66Met Polymorphism and Serum BDNF in Patients with Major Depressive Disorder (MDD).","authors":"Raja Babu Ramawat, Rizwana Quraishi, Raman Deep, Ram Kumar, Ashwani Kumar Mishra, Raka Jain","doi":"10.1177/02537176241280050","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The brain-derived neurotrophic factor (BDNF) has an important role in the growth of neurons and synaptic transmission. The BDNF gene Val66Met polymorphism (G/A) is associated with depression, but findings have not been consistently replicated. This study adopts a case-control design with an aim to investigate the association of Val66Met polymorphism and peripheral BDNF (serum) levels in patients of major depressive disorder (MDD) and healthy individuals (controls).</p><p><strong>Materials and method: </strong>This study adopts an observational, case-control design with a total of 174 participants. Cases (n = 87) were currently depressed, having Diagnostic and Statistical Manual of Mental Disorders (DSM-5) MDD, without psychiatric comorbidity. Controls (n = 87) comprised healthy individuals with no family history of psychiatric illness. The cases were evaluated using the NIMH-Life Chart Method, Hamilton Depression Rating Scale (HAM-D), Clinically Useful Depression Outcome Scale (CUDOS), and Clinical Global Impression (CGI). TaqMan assay was used for genotyping, and serum BDNF was measured using Enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>The case mean age was 35.32 ± 11.69 years (52% females) and comparable to controls. Allelic distribution was 33% (Met), and genotypic distribution was 17% (Met/Met), 32% (Val/Met), and 51% (Val/Val) for cases. The genotypic distribution did not differ across study groups. Serum BDNF was significantly lower in MDD cases as compared to controls (<i>p <</i> .001). The serum BDNF levels were comparable across the genotypic groups among cases.</p><p><strong>Conclusion: </strong>The Val66Met polymorphism has not been associated with a risk for MDD and, interestingly, did not influence the BDNF levels (serum). Significantly low BDNF levels were found in MDD cases. The study findings show that factors other than Val66Met gene polymorphism have a role in modulating serum BDNF levels.</p>","PeriodicalId":13476,"journal":{"name":"Indian Journal of Psychological Medicine","volume":" ","pages":"02537176241280050"},"PeriodicalIF":1.9000,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11572419/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Psychological Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/02537176241280050","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PSYCHIATRY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: The brain-derived neurotrophic factor (BDNF) has an important role in the growth of neurons and synaptic transmission. The BDNF gene Val66Met polymorphism (G/A) is associated with depression, but findings have not been consistently replicated. This study adopts a case-control design with an aim to investigate the association of Val66Met polymorphism and peripheral BDNF (serum) levels in patients of major depressive disorder (MDD) and healthy individuals (controls).
Materials and method: This study adopts an observational, case-control design with a total of 174 participants. Cases (n = 87) were currently depressed, having Diagnostic and Statistical Manual of Mental Disorders (DSM-5) MDD, without psychiatric comorbidity. Controls (n = 87) comprised healthy individuals with no family history of psychiatric illness. The cases were evaluated using the NIMH-Life Chart Method, Hamilton Depression Rating Scale (HAM-D), Clinically Useful Depression Outcome Scale (CUDOS), and Clinical Global Impression (CGI). TaqMan assay was used for genotyping, and serum BDNF was measured using Enzyme-linked immunosorbent assay (ELISA).
Results: The case mean age was 35.32 ± 11.69 years (52% females) and comparable to controls. Allelic distribution was 33% (Met), and genotypic distribution was 17% (Met/Met), 32% (Val/Met), and 51% (Val/Val) for cases. The genotypic distribution did not differ across study groups. Serum BDNF was significantly lower in MDD cases as compared to controls (p < .001). The serum BDNF levels were comparable across the genotypic groups among cases.
Conclusion: The Val66Met polymorphism has not been associated with a risk for MDD and, interestingly, did not influence the BDNF levels (serum). Significantly low BDNF levels were found in MDD cases. The study findings show that factors other than Val66Met gene polymorphism have a role in modulating serum BDNF levels.
期刊介绍：
The Indian Journal of Psychological Medicine (ISSN 0253-7176) was started in 1978 as the official publication of the Indian Psychiatric Society South Zonal Branch. The journal allows free access (Open Access) and is published Bimonthly. The Journal includes but is not limited to review articles, original research, opinions, and letters. The Editor and publisher accept no legal responsibility for any opinions, omissions or errors by the authors, nor do they approve of any product advertised within the journal.
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