Environmental factors and potential probiotic lineages shape the active prokaryotic communities associated with healthy Penaeus stylirostris larvae and their rearing water.

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Carolane Giraud, Nelly Wabete, Célia Lemeu, Nazha Selmaoui-Folcher, Dominique Pham, Viviane Boulo, Nolwenn Callac
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引用次数: 0

Abstract

Microbial dysbiosis is hypothesized to cause larval mass mortalities in New Caledonian shrimp hatcheries. In order to confirm this hypothesis and allow further microbial comparisons, we studied the active prokaryotic communities of healthy Penaeus stylirostris larvae and their surrounding environment during the first 10 days of larval rearing. Using daily nutrient concentration quantitative analyses and spectrophotometric organic matter analyses, we highlighted a global eutrophication of the rearing environment. We also evidenced drastic bacterial community modifications in the water and the larvae samples using Illumina HiSeq sequencing of the V4 region of the 16S rRNA gene. We confirmed that Alteromonadales, Rhodobacterales, Flavobacteriales, Oceanospirillales, and Vibrionales members formed the core bacteriota of shrimp larvae. We also identified, in the water and the larvae samples, several potential probiotic bacterial strains that could lead to rethink probiotic use in aquaculture (AEGEAN 169 marine group, OM27 clade, Ruegeria, Leisingera, Pseudoalteromonas, and Roseobacter). Finally, investigating the existing correlations between the environmental factors and the major bacterial taxa of the water and the larvae samples, we suggested that deterministic and stochastic processes were involved in the assembly of prokaryotic communities during the larval rearing of P. stylirostris. Overall, our results showed that drastic changes mostly occurred during the zoea stages suggesting that this larval phase is crucial during shrimp larval development.

环境因素和潜在的益生菌系形成了与健康的青花鱼幼体及其饲养水相关的活性原核生物群落。
据推测,微生物菌群失调会导致新加里东对虾孵化场幼体大量死亡。为了证实这一假设并进一步进行微生物比较,我们研究了健康的花纹对虾幼体及其周围环境在幼体饲养头 10 天内的活跃原核生物群落。通过每日营养浓度定量分析和分光光度有机物分析,我们发现饲养环境出现了全面富营养化。我们还利用 Illumina HiSeq 对 16S rRNA 基因 V4 区域进行测序,证明了水和幼虫样本中细菌群落的剧烈变化。我们证实,Alteromonadales、Rhodobacterales、Flavobacteriales、Oceanospirillales 和 Vibrionales 成员构成了对虾幼体的核心细菌群。我们还在水样和幼体样本中发现了几种潜在的益生菌株(AEGEAN 169 海洋组、OM27 支系、Ruegeria、Leisingera、Pseudoalteromonas 和 Roseobacter),这些菌株可能会促使人们重新思考在水产养殖中使用益生菌的问题。最后,通过研究环境因素与水样和幼体样本中主要细菌类群之间的现有相关性,我们认为在花纹鲈幼体饲养过程中,原核生物群落的形成既有决定性过程,也有随机过程。总之,我们的研究结果表明,剧烈的变化主要发生在幼体阶段,这表明幼体阶段在对虾幼体发育过程中至关重要。
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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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