Comparative extraction of antioxidant proteins from whole frogs (Rana ridibunda Pollas).

IF 2.7 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analytical Methods Pub Date : 2024-11-20 DOI:10.1039/d4ay01636j

Naziermu Dongmulati, Ahmidin Wali, Zi Yang, Yusufujiang Aili, Rexili Kelaimu, Yanhua Gao, Abulimiti Yili, Haji Akber Aisa

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Abstract

The forest frog (Rana ridibunda Pollas) is a traditional medicinal source rich in active protein compounds. In order to extract these compounds, six extraction methods were employed, including freeze-thaw and stirring techniques. Three different solvents were utilized in this process: 0.15 M sodium chloride (NaCl), 0.05 M phosphate buffer (PB), and 0.05 M phosphate-buffered saline (PBS). The objective was to identify the most effective extraction method. The extraction efficiencies, protein content, structure, and physicochemical properties of the extracts were compared. Additionally, antioxidant activity and free amino acid composition were analyzed. The highest-scoring extract, denoted as M1, obtained through freeze-thaw extraction using 0.15 M NaCl, exhibited an extraction rate of 7.79 ± 0.71% and a protein content of 60.36 ± 2.12%. M1 also showed antioxidant activity against DPPH˙, ABTS⁺˙, and ˙OH free radicals, with IC₅₀ values of 0.41, 0.41, and 0.39 mg mL^-1, respectively. The freeze-thaw extraction method utilizing 0.15 M NaCl has been identified as effective for extracting proteins from dried forest frogs, confirming their potential as a source of antioxidant proteins for scientific research and application.

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从整只青蛙（Rana ridibunda Pollas）中提取抗氧化蛋白的比较研究。

林蛙（Rana ridibunda Pollas）是一种传统药材，富含活性蛋白质化合物。为了提取这些化合物，我们采用了六种提取方法，包括冻融和搅拌技术。在此过程中使用了三种不同的溶剂：0.15 M 氯化钠（NaCl）、0.05 M 磷酸盐缓冲液（PB）和 0.05 M 磷酸盐缓冲盐水（PBS）。目的是找出最有效的提取方法。比较了提取物的提取效率、蛋白质含量、结构和理化性质。此外，还分析了抗氧化活性和游离氨基酸组成。通过使用 0.15 M NaCl 进行冻融萃取得到的得分最高的提取物为 M1，其萃取率为 7.79 ± 0.71%，蛋白质含量为 60.36 ± 2.12%。M1 对 DPPH˙、ABTS+˙和˙OH 自由基也具有抗氧化活性，IC50 值分别为 0.41、0.41 和 0.39 mg mL-1。利用 0.15 M NaCl 冻融萃取法提取林蛙干蛋白的效果显著，证实了林蛙干蛋白作为抗氧化蛋白来源的科研和应用潜力。

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