Emergence of polymyxin-resistant Yersinia enterocolitica strains in natural aquatic environments

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2024-11-20 DOI:10.1016/j.envpol.2024.125341

Shuqing Zhou, Huaran Wang, Haibei Li, Yidi Yang, Danyang Shi, Zhongwei Yang, Dong Yang, Tianjiao Chen, Junwen Li, Min Jin

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Abstract

Aquatic environments serve as ideal reservoirs for antibiotic-resistant bacteria and resistance genes. However, the presence of polymyxin-resistant Yersinia enterocolitica, the pathogen responsible for human yersiniosis, in aquatic environments remains poorly understood. Herein, we isolated polymyxin-resistant Y. enterocolitica strains from natural water for the first time. In addition to intrinsic resistance to ampicillin and cefazolin, the strains demonstrated high resistance to polymyxin B and polymyxin E. All isolates were capable of biofilm production and exerted high virulent effects in Galleria mellonella, with 90% mortality occurring within 48 h post-infection. Furthermore, whole genome sequencing identified 26 antibiotic resistance genes, including polymyxin resistance determinants (arnA and PmrF), beta-lactam resistance determinants (vatF and blaA), and 60 virulence genes such as yaxA and yaxB in Y. enterocolitica isolates. Notably, phylogenetic analysis revealed that Y. enterocolitica involved multilocus sequence types ST937 and ST631, which were clustered with strains isolated from a human in the United States or swine in China. The close relatedness to clinical isolates suggests that polymyxin-resistant Y. enterocolitica may pose considerable health risk to humans. Our findings provide evidence of the presence of polymyxin-resistant Y. enterocolitica in aquatic environments and raise concerns about health risks due to their potential high virulence.

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自然水生环境中出现耐多粘菌素的小肠结肠耶尔森菌菌株

水生环境是抗生素耐药细菌和耐药基因的理想温床。然而，人们对水生环境中存在的耐多粘菌素小肠结肠炎耶尔森菌（导致人类耶尔森菌病的病原体）仍然知之甚少。在本文中，我们首次从天然水中分离到了耐多粘菌素的小肠结肠炎耶尔森菌菌株。除了对氨苄西林和头孢唑啉具有内在抗性外，这些菌株还对多粘菌素 B 和多粘菌素 E 具有很高的抗性。所有分离株都能形成生物膜，并对水蚤具有很强的毒性，感染后 48 小时内死亡率高达 90%。此外，全基因组测序在小肠结肠炎病毒分离株中发现了 26 个抗生素耐药基因，包括多粘菌素耐药基因（arnA 和 PmrF）、β-内酰胺耐药基因（vatF 和 blaA）以及 60 个毒力基因（如 yaxA 和 yaxB）。值得注意的是，系统进化分析表明，小肠结肠炎病毒涉及多焦点序列类型 ST937 和 ST631，它们与从美国人或中国猪身上分离出的菌株聚类。与临床分离株的密切关系表明，耐多粘菌素的小肠结肠炎病毒可能会对人类健康造成相当大的威胁。我们的研究结果提供了水生环境中存在耐多粘菌素小肠结肠炎病毒的证据，并因其潜在的高致病力而引发了对健康风险的担忧。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.