Colletotrichum species associated with durian (Durio zibethinus Murray) in Hainan, China

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2024-11-14 DOI:10.1016/j.cropro.2024.107034

Huan Xu, Zheyu Zhang, Zhaoxi Zhou, Jonathan S. West, Jiabao Wang, Xueren Cao

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引用次数: 0

Abstract

Durian (Durio zibethinus Murray), the king of fruits, is an edible and economically important tropical fruit endemic to Southeast Asia. Durian is affected by Colletotrichum, which is one of the most important genera of plant pathogenic fungi, especially on tropical and subtropical crops. In this study, Colletotrichum species associated with durian in Hainan (China) which was a new region for durian cultivation were studied using phylogenetic and morphological analyses. The results of molecular identification based on internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin (TUB2) and the mating type locus MAT1-2 (ApMat) along with microscopic identification indicated that seven species from three species complexes were identified, including C. fructicola, C. siamense, C. queenslandicum and C. endophyticum from the gloeosporioides species complex; C.plurivorum and C. musicola from the orchidearum species complex, and C. gigasporum from the gigasporum species complex. Colletotrichum fructicola and C. siamense were the main Colletotrichum species associated with durian in Hainan. Pathogenicity tests showed that all seven species could infect durian leaves using a wound inoculation method but only C. fructicola, C. queenslandicum and C. endophyticum could infect using a non-wound inoculation method. The findings from this study enhance the knowledge of durian diseases and lay an essential foundation for devising effective disease management approaches. Further large-scale surveys are necessary, including sampling different sites and plant tissues, pathogenicity testing on a wider range of host genotypes and fungicide-sensitivity evaluation among and within the different Colletotrichum species collected.

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与中国海南榴莲（Durio zibethinus Murray）相关的 Colletotrichum 菌种

水果之王榴莲（Durio zibethinus Murray）是东南亚特有的一种可食用且具有重要经济价值的热带水果。榴莲受到 Colletotrichum 的侵染，Colletotrichum 是植物病原真菌中最重要的菌属之一，尤其侵染热带和亚热带作物。本研究采用系统发育和形态学分析方法，对海南（中国）（榴莲种植的新地区）与榴莲相关的 Colletotrichum 物种进行了研究。基于内部转录隔距（ITS）、甘油醛-3-磷酸脱氢酶（GAPDH）、β-微管蛋白（TUB2）和交配型基因座 MAT1-2（ApMat）的分子鉴定结果以及显微鉴定结果表明，共鉴定出 3 个种群中的 7 个种，包括 C. fructicola、C. si.C.plurivorum和C.musicola，以及C.gigasporum。在海南，与榴莲有关的 Colletotrichum fructicola 和 C. siamense 是主要的 Colletotrichum 种类。致病性试验表明，采用伤口接种法，所有 7 个种都能感染榴莲叶片，但采用非伤口接种法，只有 C. fructicola、C. queenslandicum 和 C. endophyticum 能感染榴莲叶片。这项研究的结果增进了人们对榴莲病害的了解，为制定有效的病害管理方法奠定了重要基础。有必要开展进一步的大规模调查，包括在不同地点和植物组织取样，在更广泛的宿主基因型上进行致病性测试，以及在收集到的不同 Colletotrichum 种类之间和种类内部进行杀菌剂敏感性评估。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.