Abscisic acid enhances SmAPK1-mediated phosphorylation of SmbZIP4 to positively regulate tanshinone biosynthesis in Salvia miltiorrhiza

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-11-19 DOI:10.1111/nph.20274

Ruiyan Zhu, Lulu Peng, Ying Xu, Changle Liu, Lili Shao, Tingyao Liu, Minyu Shou, Qinzhe Lin, Biao Wang, Min Shi, Guoyin Kai

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引用次数: 0

Abstract

Tanshinones, isolated from Salvia miltiorrhiza, is efficient to treat cardiovascular and cerebrovascular diseases. Abscisic acid (ABA) treatment is found to promote tanshinone biosynthesis; however, the underlying mechanism has not been fully elucidated.
A protein kinase namely SmAPK1 was identified as an important positive regulator of ABA-induced tanshinone accumulation in S. miltiorrhiza. Using SmAPK1 as bait, a basic region leucine zipper (bZIP) family transcription factor SmbZIP4 was screened from the cDNA library. Functional identification reveals that SmbZIP4 negatively regulates tanshinone biosynthesis in hairy roots and transgenic plants through directly targeting SmGGPPS and SmCYP76AK1.
SmAPK1 phosphorylates the Ser97 and Thr99 site of SmbZIP4, leading to its degradation via the 26S proteasome pathway, which is promoted by ABA-induced enhancement of SmAPK1 kinase activity. Degradation of SmbZIP4 upregulates the expression levels of SmGGPPS and SmCYP76AK1, resulting in increased tanshinone content.
Taken together, our results reveal new molecular mechanism by which SmAPK1-SmbZIP4 module plays a crucial role in ABA-induced tanshinone accumulation. This study sheds new insights in the biosynthesis of bioactive compounds in medicinal plants.

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脱落酸可增强 SmAPK1 介导的 SmbZIP4 磷酸化，从而积极调控丹参酮的生物合成

从丹参中分离出的丹参酮可有效治疗心脑血管疾病。研究发现，脱落酸（ABA）处理可促进丹参酮的生物合成，但其潜在机制尚未完全阐明。以 SmAPK1 为诱饵，从 cDNA 文库中筛选出了碱性区域亮氨酸拉链（bZIP）家族转录因子 SmbZIP4。功能鉴定显示，SmbZIP4通过直接靶向SmGGPPS和SmCYP76AK1，负向调控毛根和转基因植物中丹参酮的生物合成。SmAPK1使SmbZIP4的Ser97和Thr99位点磷酸化，导致其通过26S蛋白酶体途径降解，而ABA诱导的SmAPK1激酶活性增强促进了这种降解。SmbZIP4的降解会上调SmGGPPS和SmCYP76AK1的表达水平，从而导致丹参酮含量增加。这项研究为药用植物生物活性化合物的生物合成提供了新的视角。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.