SUMOylation of AL6 regulates seed dormancy and thermoinhibition in Arabidopsis

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-11-19 DOI:10.1111/nph.20270

Hua Jing, Wei Liu, Gao-Ping Qu, De Niu, Jing Bo Jin

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引用次数: 0

Abstract

DELAY OF GERMINATION1 (DOG1) is a critical regulator of seed dormancy and seed thermoinhibition. However, how DOG1 expression is regulated by post-translational modifications and how seeds transmit the high-temperature signal to DOG1 remain largely unknown.
ALFIN1-like 6/7 (AL6/7) was previously found to repress DOG1 expression during seed imbibition. Here, we found that AL6/7 represses seed dormancy partly by downregulating DOG1 expression. AtSIZ1, a SUMO E3 ligase, interacts with AL6 and mediates its SUMOylation mainly at the lysine 181 residue. SIZ1-mediated SUMOylation of AL6 is required for repression of DOG1 transcription and seed dormancy. SUMOylation of AL6 is required for its association with the DOG1 locus and protects it from ubiquitination and subsequent 26S proteasome-mediated protein degradation.
High temperatures decrease SUMOylation levels of AL6, resulting in downregulation of AL6 protein levels and an increase in DOG1 transcription, which consequently causes reduced seed germination.
Taken together, these results demonstrate that reversible SUMOylation of AL6 fine-tunes DOG1 expression, which is required for precise establishment of seed dormancy and inhibition of seed germination under high-temperature conditions in Arabidopsis thaliana.

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AL6 的 SUMOylation 调节拟南芥的种子休眠和热抑制作用

延迟种子退化1（DOG1）是种子休眠和种子热抑制的关键调节因子。然而，DOG1 的表达如何受到翻译后修饰的调控，以及种子如何将高温信号传递给 DOG1，这些问题在很大程度上仍然是未知的。在这里，我们发现 AL6/7 部分通过下调 DOG1 的表达来抑制种子休眠。AtSIZ1是一种SUMO E3连接酶，它与AL6相互作用，并主要在赖氨酸181残基上介导其SUMO化。SIZ1 介导的 AL6 SUMOylation 是抑制 DOG1 转录和种子休眠所必需的。高温会降低 AL6 的 SUMOylation 水平，导致 AL6 蛋白水平下调和 DOG1 转录增加，从而导致种子萌发率降低。综上所述，这些结果表明，AL6 的可逆 SUMO 化对 DOG1 的表达进行了微调，而 DOG1 的表达是拟南芥在高温条件下精确建立种子休眠和抑制种子萌发所必需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.