3D-Printed Microfluidic Device for Cerebrospinal Fluid Diversion: Design, Characterization, and In Vitro Evaluation of an Alternative Shunting Device

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-11-19 DOI:10.1016/j.snb.2024.136961

Seunghyun Lee, Leandro Castañeyra-Ruiz, Sora Sato, Gio Jison, Brian Hanak, Jenna Ledbetter, Amirhossein Shahriari, Celine Thao-Quyen Tran, Michael Le, Michael Muhonen

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引用次数: 0

Abstract

Hydrocephalus is a neurological disorder characterized by the accumulation of cerebrospinal fluid (CSF) in the brain, resulting in increased intracranial pressure and potentially life-threatening complications. Conventional treatment is CSF shunt implantation but, particularly in pediatric patients, this is fraught with high failure rates of 40% at 2 years and 98% at 10 years. In pursuit of improving current treatment of communicating hydrocephalus, we developed a 3D-printed microfluidic chip that uses flexible material and features a one-way valve to prevent backflow. To evaluate chip performance, we measured flow rate and differential pressure in vitro. We also cultured astrocytes on the device to assess the potential for cellular attachment. The results revealed minimal cellular attachment and absence of obstruction. The ability of this microfluidic chip to effectively regulate CSF flow highlights the potential of microfluidic technology and demonstrates this device’s capacity to serve as a model for the next generation of CSF shunt devices.

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用于脑脊液分流的三维打印微流控装置：替代分流装置的设计、表征和体外评估

脑积水是一种神经系统疾病，其特点是脑脊液（CSF）在大脑中积聚，导致颅内压增高，并可能引发危及生命的并发症。传统的治疗方法是植入脑脊液分流术，但尤其对儿童患者而言，这种方法的失败率很高，2 年内的失败率为 40%，10 年内的失败率为 98%。为了改善目前对交流性脑积水的治疗，我们开发了一种三维打印的微流体芯片，它使用柔性材料，具有单向阀以防止回流。为了评估芯片的性能，我们在体外测量了流速和压差。我们还在该装置上培养了星形胶质细胞，以评估细胞附着的可能性。结果显示，细胞附着极少，也没有阻塞。这种微流控芯片能够有效调节 CSF 流量，凸显了微流控技术的潜力，并证明了该装置有能力作为下一代 CSF 分流装置的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.