Transcriptomic and metabolomic analysis reveal differentially expressed genes and metabolic pathways in bermudagrass under drought stress

IF 2 3区农林科学 Q2 AGRONOMY

Crop Science Pub Date : 2024-11-18 DOI:10.1002/csc2.21412

Ravi Teja Seelam, David Jespersen

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引用次数: 0

Abstract

Drought stress poses a significant challenge to turfgrass growth, particularly in the regions like southern United States, where bermudagrass (Cynodon sp.) is widely used for lawns and sports fields. Drought stress disrupts physiological processes, leading to reduced water availability, impaired photosynthesis, and oxidative stress. To understand the bermudagrass response to drought, we investigated the physiological differences and characterized the gene expression and metabolite profiles in two bermudagrass genotypes, TifTuf and Premier. Physiological measurements showed significant variations in green cover percentage, visual quality, and relative water content between the two genotypes. RNA sequencing revealed extensive gene expression changes, with differentially expressed genes that were upregulated in both genotypes. Gene ontology (GO) analysis highlighted biological processes such as transcription regulation, lipid metabolism, and cellular structure development pathways. KEGG pathway analysis indicated that TifTuf had significant changes in galactose metabolism, carotenoid biosynthesis, and plant hormone signal transduction pathways, while Premier showed enrichment in plant hormone signaling, lipid metabolism, and secondary metabolite biosynthesis pathways. Metabolomic analysis provided insights into metabolic reprogramming due to drought stress. Principal component analysis revealed distinct metabolic patterns between control and drought-stressed samples, with both genotypes showing substantial alterations. Differential metabolite analysis identified key metabolites associated with stress adaptation, including the phytohormone ABA and various amino acids. This analysis elucidates the intricate physiological and molecular mechanisms underlying drought tolerance in bermudagrass genotypes. These findings enhance the understanding of drought stress adaptation strategies in bermudagrass and offer valuable insights for the development of drought-tolerant genotypes.

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转录组和代谢组分析揭示了干旱胁迫下百慕大草的差异表达基因和代谢途径

干旱胁迫对草坪草的生长构成了巨大挑战，尤其是在美国南部等地区，百慕大草（Cynodon sp.）被广泛用于草坪和运动场。干旱胁迫会扰乱生理过程，导致水分供应减少、光合作用受损和氧化应激。为了了解百慕大草对干旱的反应，我们研究了两种百慕大草基因型 TifTuf 和 Premier 的生理差异，并分析了基因表达和代谢物特征。生理测量结果表明，两种基因型在绿色覆盖率、视觉质量和相对含水量方面存在显著差异。RNA 测序显示了广泛的基因表达变化，两种基因型中的差异表达基因都出现了上调。基因本体（GO）分析强调了转录调控、脂质代谢和细胞结构发育途径等生物过程。KEGG 通路分析表明，TifTuf 在半乳糖代谢、类胡萝卜素生物合成和植物激素信号转导通路上有显著变化，而 Premier 则在植物激素信号转导、脂质代谢和次生代谢物生物合成通路上表现出富集。代谢组分析深入揭示了干旱胁迫导致的代谢重编程。主成分分析揭示了对照样本和干旱胁迫样本之间不同的代谢模式，两种基因型都发生了重大变化。差异代谢物分析确定了与胁迫适应相关的关键代谢物，包括植物激素 ABA 和各种氨基酸。这项分析阐明了百慕大草基因型耐旱性背后错综复杂的生理和分子机制。这些发现加深了人们对百慕大草干旱胁迫适应策略的理解，并为开发耐旱基因型提供了宝贵的见解。

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来源期刊

Crop Science 农林科学-农艺学

CiteScore

4.50

自引率

8.70%

发文量

197

审稿时长

3 months

期刊介绍： Articles in Crop Science are of interest to researchers, policy makers, educators, and practitioners. The scope of articles in Crop Science includes crop breeding and genetics; crop physiology and metabolism; crop ecology, production, and management; seed physiology, production, and technology; turfgrass science; forage and grazing land ecology and management; genomics, molecular genetics, and biotechnology; germplasm collections and their use; and biomedical, health beneficial, and nutritionally enhanced plants. Crop Science publishes thematic collections of articles across its scope and includes topical Review and Interpretation, and Perspectives articles.