BoaCRTISO regulates the color and glossiness of Chinese kale through its effects on pigment, abscisic acid, and cuticular wax biosynthesis

IF 5.7 1区农林科学 Q1 HORTICULTURE

Horticultural Plant Journal Pub Date : 2024-11-12 DOI:10.1016/j.hpj.2024.06.008

Chenlu Zhang, Ling Li, Yutong Zhang, Qiannan Liang, Sha Luo, Zhi Huang, Huanxiu Li, Victor Hugo Escalona, Zhifeng Chen, Fen Zhang, Yi Tang, Bo Sun

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引用次数: 0

Abstract

Carotenoid isomerase (CRTISO) is an important enzyme in carotenoid biosynthesis, and it catalyzes the conversion of lycopene precursors to lycopene in several plant species. However, the role of CRTISO in other biochemical processes during plant growth and development remains unclear. Here, we showed that Chinese kale boacrtiso mutants have distinctive characteristics, including a yellow-green hue and glossy appearance, and this contrasts with the dark green and glaucous traits observed in wild-type (WT) plants. Analysis of pigments in mutants revealed that the reduction in the content of carotenoids and chlorophylls contributed to the yellow-green coloration observed in mutants. An examination of cuticular waxes in Chinese kale indicated that there was a decrease in both the total wax content and the content of individual waxes in boacrtiso mutants (bearing a mutation of BoaCRTISO), which may be caused by the decrease of abscisic acid (ABA) content. The expression of carotenoid, chlorophyll, ABA, and wax biosynthesis genes was down-regulated in boacrtiso mutants. This finding confirms that BoaCRTISO regulates the biosynthesis of pigments, ABA, and cuticular waxes in Chinese kale. Our results provide new insights into the interplay between plant pigment and cuticular wax metabolic pathways in Brassica vegetables.

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BoaCRTISO 通过影响色素、脱落酸和角质蜡的生物合成来调节甘蓝的颜色和光泽度

类胡萝卜素异构酶（CRTISO）是类胡萝卜素生物合成过程中的一种重要酶，在多种植物中催化番茄红素前体向番茄红素的转化。然而，CRTISO 在植物生长发育过程中的其他生化过程中的作用仍不清楚。在这里，我们发现中国羽衣甘蓝boacrtiso突变体具有独特的特征，包括黄绿色调和有光泽的外观，这与在野生型（WT）植物中观察到的深绿色和有白霜的特征形成鲜明对比。对突变体色素的分析表明，类胡萝卜素和叶绿素含量的减少导致突变体呈现黄绿色。对芥蓝角质层蜡质的研究表明，boacrtiso 突变体（BoaCRTISO 基因突变）的总蜡质含量和单个蜡质含量都有所下降，这可能是脱落酸（ABA）含量减少所致。在 Boacrtiso 突变体中，类胡萝卜素、叶绿素、ABA 和蜡质生物合成基因的表达下调。这一发现证实 BoaCRTISO 调控羽衣甘蓝中色素、ABA 和角质蜡的生物合成。我们的研究结果为了解甘蓝类蔬菜中植物色素和角质蜡代谢途径之间的相互作用提供了新的视角。

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来源期刊

Horticultural Plant Journal Environmental Science-Ecology

CiteScore

9.60

自引率

14.00%

发文量

293

审稿时长

33 weeks

期刊介绍： Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.