The impact of glutamine synthetase PbgsA on the growth, conidiation and mycophenolic acid production of Penicillium brevicompactum

IF 2.4 3区生物学 Q3 GENETICS & HEREDITY

Fungal Genetics and Biology Pub Date : 2024-11-16 DOI:10.1016/j.fgb.2024.103941

TingTing Hu , Jishou Wu , Zixuan Lin , Yi Lin , Lin Lin , Wei Wei , Dongzhi Wei

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Abstract

Glutamine synthetase (GS) is a critical enzyme in nitrogen metabolism regulation and plays an essential role in the metabolic pathways involved in microbial growth and development. Penicillium brevicompactum, known for its rich repertoire of secondary metabolites, including mycophenolic acid (MPA), lacks research on the regulatory mechanisms of GS within this species. This study aimed to investigate the influence of GS on the growth, sporulation, and secondary metabolism of P. brevicompactum to elucidate the biological function of GS in this organism. We identified the glutamine synthetase gene (PbgsA) from P. brevicompactum and constructed PbgsA gene-overexpression and gene-silencing transformants. The impact of PbgsA on growth and sporulation was evaluated, revealing that PbgsA gene-overexpression transformants exhibited enhanced growth and significantly increased the expression levels of sporulation pathway genes (brlA, abaA, and wetA). Additionally, PbgsA gene-overexpression transformants produced higher MPA yields, with a maximum of 4.78 g/L, representing a 54.19 % increase compared to the wild type (WT). Conversely, PbgsA gene-silencing transformants showed reduced MPA production, with a minimum yield of 1.13 g/L, a 63.55 % decrease relative to the WT. Transcriptional analysis of the MPA biosynthetic gene cluster indicated that PbgsA exerted regulatory effects on certain biosynthetic pathway genes, such as mpaA and mpaB. This study demostrated the potential positive regulatory role of glutamine synthetase PbgsA in the growth, spore development, and secondary metabolism of P. brevicompactum, which provided a new strategy for genetic regulation in filamentous fungal.

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谷氨酰胺合成酶 PbgsA 对破伤风青霉的生长、分生孢子和产生霉酚酸的影响。

谷氨酰胺合成酶（GS）是氮代谢调节中的一种关键酶，在微生物生长发育的代谢途径中发挥着重要作用。布氏青霉（Penicillium brevicompactum）以其丰富的次生代谢产物（包括霉酚酸（MPA））而闻名，但对该菌种内 GS 的调控机制缺乏研究。本研究旨在调查 GS 对 P. brevicompactum 的生长、孢子和次生代谢的影响，以阐明 GS 在该生物体内的生物学功能。我们确定了 P. brevicompactum 的谷氨酰胺合成酶基因（PbgsA），并构建了 PbgsA 基因外表达和基因沉默转化子。结果表明，PbgsA 基因缺失表达转化子的生长能力增强，孢子途径基因（brlA、abaA 和 wetA）的表达水平显著提高。此外，PbgsA 基因缺失转化体产生了更高的 MPA 产量，最高达 4.78 克/升，与野生型（WT）相比增加了 54.19%。相反，PbgsA 基因沉默转化体的 MPA 产量降低，最低产量为 1.13 克/升，比 WT 降低了 63.55%。对 MPA 生物合成基因簇的转录分析表明，PbgsA 对某些生物合成途径基因（如 mpaA 和 mpaB）具有调控作用。该研究证明了谷氨酰胺合成酶PbgsA在P. brevicompactum的生长、孢子发育和次生代谢中的潜在正向调控作用，为丝状真菌的遗传调控提供了一种新策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fungal Genetics and Biology 生物-遗传学

CiteScore

6.20

自引率

3.30%

发文量

审稿时长

85 days

期刊介绍： Fungal Genetics and Biology, formerly known as Experimental Mycology, publishes experimental investigations of fungi and their traditional allies that relate structure and function to growth, reproduction, morphogenesis, and differentiation. This journal especially welcomes studies of gene organization and expression and of developmental processes at the cellular, subcellular, and molecular levels. The journal also includes suitable experimental inquiries into fungal cytology, biochemistry, physiology, genetics, and phylogeny. Fungal Genetics and Biology publishes basic research conducted by mycologists, cell biologists, biochemists, geneticists, and molecular biologists. Research Areas include: • Biochemistry • Cytology • Developmental biology • Evolutionary biology • Genetics • Molecular biology • Phylogeny • Physiology.