Lactobacillus murinus alleviates insulin resistance via promoting L-citrulline synthesis.

IF 5.4 2区医学 Q1 Medicine

Journal of Endocrinological Investigation Pub Date : 2024-11-19 DOI:10.1007/s40618-024-02500-5

Jianglan Long, Zhe Shi, Zenghui Miao, Linjie Dong, Dan Yan

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引用次数: 0

Abstract

Aims: The role of Lactobacillus murinus as a potential probiotic is being explored. Our objectives were to explore the effects of Lactobacillus murinus on insulin resistance and the underlying mechanism.

Methods: Insulin resistance animal models were applied to study the effect of L. murinus and the underlying mechanism by six weeks of treatment. Metformin was administered in vitro to analyze the growth and metabolites of L. murinus. Serum metabolites were further analyzed after L. murinus administration. The effect of L-citrulline and the underlying mechanism in alleviating insulin resistance were evaluated.

Results: L. murinus not only reduced body weight gain and postprandial blood glucose (PBG) but improved impaired glucose tolerance (IGT) and insulin resistance. Moreover, L. murinus inhibited the secretion of pro-inflammatory factors (IL-1β, IL-6 and TNF-α) while promoted the secretion of anti-inflammatory factor (IL-10). Further, L. murinus promoted the expression of carnitine palmitoyl transferase 1 (CPT1) while inhibited phosphoenolpyruvate carboxykinase (PCK) and glucose-6-phosphatase (G6Pase). A total of 147 metabolites of L. murinus were identified, in which the content of L-citrulline increased to 7.94 times after metformin regulation. Further, the serum concentration of L-citrulline significantly increased after L. murinus administration. Similarly, L-citrulline reduced body weight gain and PBG, improved IGT and insulin resistance. Additionally, L-citrulline improved inflammation, promoted CPT1 while inhibited PCK and G6Pase.

Conclusions: L. murinus mediated by L-citrulline alleviated insulin resistance via promoting fatty acid oxidation and inhibiting gluconeogenesis, suggesting that supplementation of L. murinus could be a potential therapeutic approach for type 2 diabetes related to insulin resistance.

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鼠乳杆菌通过促进 L-瓜氨酸的合成缓解胰岛素抵抗。

目的：人们正在探索鼠乳杆菌作为一种潜在益生菌的作用。我们的目标是探索鼠乳杆菌对胰岛素抵抗的影响及其内在机制：方法：应用胰岛素抵抗动物模型，通过六周的治疗研究鼠乳杆菌的作用及其内在机制。体外给药二甲双胍以分析L.murinus的生长和代谢产物。对服用L. murinus后的血清代谢物进行了进一步分析。评估了L-瓜氨酸在缓解胰岛素抵抗方面的作用及其内在机制：结果：L. murinus不仅降低了体重增加和餐后血糖（PBG），还改善了糖耐量受损（IGT）和胰岛素抵抗。此外，L. murinus 还能抑制促炎因子（IL-1β、IL-6 和 TNF-α）的分泌，同时促进抗炎因子（IL-10）的分泌。此外，L. murinus 还能促进肉碱棕榈酰转移酶 1（CPT1）的表达，同时抑制磷酸烯醇丙酮酸羧激酶（PCK）和葡萄糖-6-磷酸酶（G6Pase）。共鉴定出 147 种 L. murinus 代谢物，其中 L-瓜氨酸的含量在二甲双胍调节后增加到 7.94 倍。此外，服用L. murinus后，血清中的L-瓜氨酸浓度明显增加。同样，L-瓜氨酸可降低体重增加和 PBG，改善 IGT 和胰岛素抵抗。此外，L-瓜氨酸还能改善炎症，促进 CPT1，同时抑制 PCK 和 G6Pase：结论：L. murinus通过促进脂肪酸氧化和抑制葡萄糖生成，缓解了胰岛素抵抗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Endocrinological Investigation ENDOCRINOLOGY & METABOLISM-

CiteScore

8.10

自引率

7.40%

发文量

242

期刊介绍： The Journal of Endocrinological Investigation is a well-established, e-only endocrine journal founded 36 years ago in 1978. It is the official journal of the Italian Society of Endocrinology (SIE), established in 1964. Other Italian societies in the endocrinology and metabolism field are affiliated to the journal: Italian Society of Andrology and Sexual Medicine, Italian Society of Obesity, Italian Society of Pediatric Endocrinology and Diabetology, Clinical Endocrinologists’ Association, Thyroid Association, Endocrine Surgical Units Association, Italian Society of Pharmacology.