Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera: signalling pathways involved.

IF 1.5 4区生物学 Q4 CELL BIOLOGY

Zygote Pub Date : 2024-12-01 Epub Date: 2024-11-19 DOI:10.1017/S096719942400042X

Maria Fernanda Medina, Ana Pucci, Franco José Pucci, Marta Ines Bühler, María Cecilia Gramajo-Bühler

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引用次数: 0

Abstract

The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of Chichilla lanigera exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes. We determined the participation of different cell signalling pathways in the induced acrosome reaction. Our results showed an alteration in the progressive motility pattern and cell viability depending on DhL concentration and exposure time of gametes. When analyzing acrosomal status, higher percentages than the negative control were obtained in all tested doses. Both isolated and joint inhibition tests of PKA and phospholipases (PLC and PLA₂) showed a greater participation of PI-PLC. This is the first report concerning the effects of this lactone on the medium of sperm incubation. Consequently, further studies will be necessary to determine the molecular implications of this lactone on the fertilizing potential of the sperm.

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脱氢亮氨酸（DhL）对栗鼠精子顶体反应的影响：相关信号通路。

30 多年来，不同的研究小组一直在研究多种植物的次级代谢产物，特别是倍半萜内酯（SLs）。这类代谢物具有多种生物活性，如抗菌、抗病毒、抗溃疡、细胞增殖抑制剂和卵母细胞激活剂，并参与外泌过程。本研究旨在评估在 10 至 40 分钟的不同孵育时间内暴露于浓度不断增加（0 至 2 mM）的 DhL 中的 Chichilla lanigera 附睾配子的一些精子参数。我们确定了不同细胞信号通路在诱导顶体反应中的参与情况。我们的结果表明，配子的渐进运动模式和细胞活力的改变取决于 DhL 的浓度和暴露时间。在分析顶体状态时，所有测试剂量下的顶体率都高于阴性对照。对 PKA 和磷脂酶（PLC 和 PLA2）的单独和联合抑制测试表明，PI-PLC 的参与程度更高。这是第一份有关这种内酯对精子培养基影响的报告。因此，有必要进行进一步研究，以确定这种内酯对精子受精潜能的分子影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zygote 生物-发育生物学

CiteScore

1.70

自引率

5.90%

发文量

117

审稿时长

6-12 weeks

期刊介绍： An international journal dedicated to the rapid publication of original research in early embryology, Zygote covers interdisciplinary studies on gametogenesis through fertilization to gastrulation in animals and humans. The scope has been expanded to include clinical papers, molecular and developmental genetics. The editors will favour work describing fundamental processes in the cellular and molecular mechanisms of animal development, and, in particular, the identification of unifying principles in biology. Nonetheless, new technologies, review articles, debates and letters will become a prominent feature.